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Research Article

Isolation and Molecular Identification of Auxotrophic Mutants to Develop a Genetic Manipulation System for the Haloarchaeon Natrinema sp. J7-2

Table 4

Molecular characterization of the auxotrophic mutants by analyzing relevant genes in the corresponding amino acid biosynthetic pathways.

Auxotrophic strainsPhenotypeGeneGene numberNotationLength (bp)Mutation status

J7-2-1LeuleuA NJ7_G12722-Isopropylmalate synthase831None
leuC NJ7_G17483-Isopropylmalate dehydratase, large subunit1388None
leuD NJ7_G17493-Isopropylmalate dehydratase, small subunit837None
leuB NJ7_G17523-Isopropylmalate dehydrogenase 76812 bp fragment deletion from 690 to 701 bp

J7-2-26LysdapA NJ7_G4051Dihydrodipicolinate synthase 951None
dapF NJ7_G4046Diaminopimelate epimerase 1639None
lysA NJ7_G4047Diaminopimelate decarboxylase 1422None
dapD NJ7_G40492,3,4,5-Tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase63518 bp deletion from 583 to 600 bp
dapB NJ7_G4050Dihydrodipicolinate reductase 987None

Auxotrophic strainsPhenotypeGeneGene numberNotationLength (bp) Mutation Status
J7-2-22J7-2-52

J7-2-22
J7-2-52
ArgargF NJ7_G0258Ornithine carbamoyltransferase 921NoneNone
argE NJ7_G0259N-Acetyl-ornithine/N-acetyl-lysine deacetylase 1110NoneNone
argD NJ7_G0260Acetylornithine aminotransferase1155NoneNone
argB NJ7_G0261Acetylglutamate kinase 870NoneNone
argC NJ7_G0262N-Acetyl-gamma-glutamyl-phosphate reductase 1077None1051 bp: G-A(GGG-GAG)
argX NJ7_G0263Ribosomal protein S6 modification protein1043NoneNone
argW NJ7_G0264Putative biosynthetic carrier protein
argH NJ7_G0265Argininosuccinate lyase1539NoneNone
argG NJ7_G0266Argininosuccinate synthase 1242NoneNone

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