Research Article

Biochemical Characterisation of Phage Pseudomurein Endoisopeptidases PeiW and PeiP Using Synthetic Peptides

Figure 3

Purification samples of PeiW and PeiP on SDS-PAGE. Gel (a) PeiW; lane 1, molecular weight markers with sizes indicated in kDa, lane 2, PeiW crude lysate supernatant, and lane 3, PeiW after nickel-affinity chromatography step. Gel (b) PeiP; lane 1, molecular weight markers with sizes indicated in kDa, lane 2, PeiP crude lysate supernatant, and lane 3, PeiP after desalting step. A vertical line in each gel indicates the removal of irrelevant lanes.
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