Biochemical Characterisation of Phage Pseudomurein Endoisopeptidases PeiW and PeiP Using Synthetic Peptides

Figure 4

Activity of (a) PeiW and (b) PeiP in aerobic and anaerobic cell suspension assays. The assays were performed using Mtb. thermautotrophicus ΔH cells, as described in the Materials and Methods, ● aerobic, ○ anaerobic. Initial OD₆₀₀ values were 0.771, PeiW aerobic; 0.865, PeiW anaerobic; 1.014, PeiP aerobic; 0.940, PeiP anaerobic. The total OD change of the enzyme-free and cell-free controls was less than 2% of the initial value.

(a)

(b)