Biochemical Characterisation of Phage Pseudomurein Endoisopeptidases PeiW and PeiP Using Synthetic Peptides

Table 2

Substrate specificity, activity of PeiW or PeiP on synthetic peptide substrates using an agarose plate assay. The intensity of a zone of yellow colouration was compared; − no zone, + zone being only just observable, and +++++ very large zone. No yellow zones were observed with control buffer in the wells.

Synthetic peptide substrate	Abbreviation	Relative activity
		PeiW	PeiP
L-Ala-pNA	ApNA	+	+
H-Glu-Ala-pNA	EApNA	+++	++
Glu--Ala-pNA (Glu(Ala-pNA)-OH)	EApNA	+++++	+++++
Glu--Ser-pNA (Glu(Ser-pNA)-OH)	ESpNA	+++	++
Glu--Thr-pNA (Glu(Thr-pNA)-OH)	ETpNA	−	−
Asp--Ala-pNA (Asp(Ala-pNA)-OH)	DApNA	+	+
L-Glu--pNA (Glu(pNA)-OH)	EpNA	+	−