Research Article

Biochemical Characterisation of Phage Pseudomurein Endoisopeptidases PeiW and PeiP Using Synthetic Peptides

Table 3

Substrate specificity, activity of PeiW or PeiP on methanogen cells as substrates using an agarose plate lysate assay. Activity is diameter (mm) of clear zone produced in agarose plate. No clear zone is indicated by −. The diameter of the well was 4 mm. No clear zones were observed with control buffer in the wells.

Methanogen cell substrateActivity, diameter of clear zone (mm)
PeiWPeiP

Methanobacterium formicicum strain BRM92218
Methanosphaera stadtmanae (DSM 3091)1920
Methanothermobacter thermautotrophicus  H1918
Methanobrevibacter ruminantium strain 31A2116
Methanobrevibacter smithii (DSM 861)2017
Methanobrevibacter gottschalkii (DSM 11977)2012
Methanobrevibacter sp. SM910
Methanobrevibacter ruminantium strain M1
Methanosarcina barkeri strain CM1
Methanospirillum hungatei (DSM 864)
No cells (buffer control)