Characterization of the ATP-Dependent Lon-Like Protease in Methanobrevibacter smithii

Figure 1

Phylogenetic and sequence analysis of Lon-like-Ms. (a) Unrooted neighbor-joining phylogenetic tree of Lon from the LonA (yellow), LonB (blue), and Lon-like (green) families, generated using MEGA5. The optimal tree with a branch length sum of 13.47550492 is shown. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) is shown next to the branches. (b) Lon-like-Ms and protease Lon sequences from T. kodakarensis KOD1, E. coli, and yeast were aligned. The Walker A, Walker B, and the possible active site are shown in the rectangles. The conservation level of each residue is indicated by the height of the bars above each residue. The number at the ending of each line of amino acids indicates the number of the amino acid residues. (c) Putative domains of Lon proteases from M. smithii (Lon-like-Ms and WP_011954752), E. coli (Lon-Ec, WP_001295325.1), yeast (Lon-Sc, NP_009531.1), human (Lon-Homo, NP_004784.2), and T. kodakarensis (Lon-Tk, WP_011250215.1).

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