Research Article
Characterization of the ATP-Dependent Lon-Like Protease in Methanobrevibacter smithii
Table 1
Relative cleavage activities of Lon-like-Ms in the presence of different nucleotides or divalent cations.
| Nucleotide | Divalent cation | Peptide hydrolysis (%) | Casein hydrolysis (%) |
| None | None | 5 | 7 | ATP | Mg2+ | 100 | 100 | ATP | None | 6 | 4 | None | Mg2+ | 10 | 8 | AMP-PNP | Mg2+ | 92 | 80 | ADP | Mg2+ | 64 | 72 | GTP | Mg2+ | 90 | 85 | CTP | Mg2+ | 131 | 122 | UTP | Mg2+ | 64 | 55 | ATP | Ni2+ | 20 | 16 | ATP | Ca2+ | 83 | 80 | ATP | Mn2+ | 89 | 72 | ATP | Co2+ | 70 | 65 | ATP | Zn2+ | 12 | 9 |
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