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Volume 2016, Article ID 5916067, 10 pages
Research Article

Methanogen Diversity in Indigenous and Introduced Ruminant Species on the Tibetan Plateau

1School of Public Health, Lanzhou University, 222 Tianshui Nanlu, Lanzhou 730000, China
2School of Life Science, Lanzhou University, 222 Tianshui Nanlu, Lanzhou 730000, China
3CSIRO, Agriculture Flagship, Queensland Bioscience Precinct, 306 Carmody Road, St Lucia, QLD 4067, Australia

Received 11 February 2016; Accepted 11 April 2016

Academic Editor: Hans-Peter Klenk

Copyright © 2016 Xiao Dan Huang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The Shannon index analysis indicated a higher diversity among Tibetan sheep (5.76±0.04) and yak (5.70±0.04) (P<0.05) compared to crossbred sheep (5.37±0.02) and cattle (5.49±0.07) (Supplementary Figure 1).

The beta diversity measures for the comparison of the rumen methanogen community structure showed a separation from the small ruminant and large ruminant groups explained by the first axis of variance (25.5%), while a smaller percentage of variance was observed from the cattle and yak samples along the second axis (16.1%) (Supplementary Figure 2).

Analysis of changes in the abundance of these OTUs for specific animal pairwise comparisons is presented in the Supplementary Figures and statistically confirms the observations shown in Figure 2 (Supplementary Figures 3–8 for further details). Furthermore OTUs common to all animals as indicated in Figure 2 were not found to be significantly different in pairwise comparisons (Supplementary Figures 3–8).

  1. Supplementary Material