Unusually High Archaeal Diversity in a Crystallizer Pond, Pomorie Salterns, Bulgaria, Revealed by Phylogenetic Analysis

Figure 1

RFLP analysis of the retrieved clones. (a) Agarose gel electrophoresis of products after restriction with Msp I; 1–6, 8–12: restriction patterns; 7: marker Gene Ruler 50 bp (Fermentas). (b) Agarose gel electrophoresis after restriction with Hae III of the sequences with the same Msp I restriction pattern; 1–6, 8–14: restriction patterns; 7: marker Gene Ruler 50 bp (Fermentas). Additional splitting of the group was observed. (c) Rarefaction curve for sampling of PS 16S rRNA archaeal gene library. Rarefaction curve was calculated using Analytical Rarefaction version 1.3 (http://www.uga.edu/strata/software/index.html).

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