Characterization of a Thermostable 8-Oxoguanine DNA Glycosylase Specific for GO/N Mismatches from the Thermoacidophilic Archaeon<i> Thermoplasma volcanium</i>

<div>Purification of TvoOgg recombinant protein from<i> E. coli</i>. A Coomassie brilliant blue stained SDS-polyacrylamide gel (15%) showing separation of a lysate of BL21 (DE3) cells containing pET28a-TvoOgg after induction by 1 mM IPTG for 5 hr (lane 1), eluted fractions of TvoOgg from Ni-NTA agarose columns (lane 2–9), or SP Sepharose FF columns (lane 10–14), respectively, and TvoOgg protein after dialysis against dialysis buffer 1 (lane 15). The TvoOgg protein is indicated by an arrow. Lane M contained molecular mass standards (Bio-Rad, U.S.A.) as indicated on the left.</div>

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Figure 2

Figure 2: Characterization of a Thermostable 8-Oxoguanine DNA Glycosylase Specific for GO/N Mismatches from the Thermoacidophilic Archaeon<i> Thermoplasma volcanium</i> 

Figure 2 | Characterization of a Thermostable 8-Oxoguanine DNA Glycosylase Specific for GO/N Mismatches from the Thermoacidophilic Archaeon Thermoplasma volcanium 