Characterization of a Thermostable 8-Oxoguanine DNA Glycosylase Specific for GO/N Mismatches from the Thermoacidophilic Archaeon Thermoplasma volcanium

Figure 6

Time course of TvoOgg activity on substrates , , , and . One pmol of TvoOgg was incubated with heteroduplex DNA containing , , , and mismatches at 60°C. At the indicated time points, the reaction was quenched with 4 μL of 1 M NaOH to inactivate enzyme and cleave resulting AP sites. The uncut 34-mer DNA substrates (S) and cleaved 13-mer products (P) are indicated on the left. Asterisks indicate 5′-FAM-labeled strand. Each data point is represented as the mean ± SD of three independent measurements.

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