Research Article

NADP-Dependent Aldehyde Dehydrogenase from Archaeon Pyrobaculum sp.1860: Structural and Functional Features

Figure 5

Different conformations of the coenzyme in the complexes with AlDHPyr1147. Coenzyme is in magenta, and AlDHPyr1147 is in green. (a) The coenzyme is in two conformations in the Holo-1 and Holo-2 complexes. The omit electron density map for the model Holo-2 around the coenzyme is contoured at 2.7σ and colored green. (b) NADP+ is in the “hydride transfer” conformation in the Holo-3 model. The omit electron density map for the model Holo-3 around the coenzyme is contoured at 3σ and colored green. ((c) and (d)) The electron density maps for the AlDHPyr1147 model contoured at 1σ and colored blue. Hydrogen bonds are shown by dashed lines. (c) An enlarged view of coenzyme in the “hydride transfer” conformation in the active site of the Holo-1 model. (d) An enlarged view of coenzyme in the “out” conformation in the Holo-1 model.
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