NADP-Dependent Aldehyde Dehydrogenase from Archaeon Pyrobaculum sp.1860: Structural and Functional Features

Table 3

Kinetic parameters of AlDHPyr1147 in reactions with different aldehydes. Conditions: 50 mM sodium pyrophosphate buffer, pH 8.8, 100 mM NaCl, and 0.3 mM NADP+, 60°C.

Substrate	, s^−1	, M	/, s^−1 M^−1
Isobutyraldehyde	4.1 ± 0.4	0.020 ± 0.003	200 ± 50
Propionaldehyde	2.9 ± 0.16	0.021 ± 0.0025	140 ± 30
Glyceraldehyde	1.2 ± 0.2	0.015 ± 0.006	80 ± 40