Research Article

Growth Characteristics of Methanomassiliicoccus luminyensis and Expression of Methyltransferase Encoding Genes

Figure 5

Ratios of transcript abundances of genes encoding corrinoid proteins involved in the degradation of methylamines in M. luminyensis. The abundance of a certain gene transcript was compared to its abundance during growth on methanol. ΔCT values were calculated using the relative amount of reference gene transcripts as a standard (CT gene—CT S15). ΔΔCT values derived from the ΔCT values of genes of interest in methanol-grown cultures minus the ΔCT values of genes of interest in TMA- (a), DMA- (b), or MMA- (c) grown cultures. Ratios were calculated from ΔΔCT values by using the function 2ΔΔCT.