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Volume 2017 (2017), Article ID 4097425, 9 pages
https://doi.org/10.1155/2017/4097425
Research Article

The Draft Genome of the Non-Host-Associated Methanobrevibacter arboriphilus Strain DH1 Encodes a Large Repertoire of Adhesin-Like Proteins

1Genomic and Applied Microbiology & Göttingen Genomics Laboratory, Georg-August University, Göttingen, Germany
2Temasek Life Sciences Laboratory, Singapore
3Department of Biological Sciences, National University of Singapore, Singapore 117604

Correspondence should be addressed to Henning Seedorf

Received 20 January 2017; Accepted 22 March 2017; Published 28 May 2017

Academic Editor: Stefan Spring

Copyright © 2017 Anja Poehlein et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Methanobrevibacter arboriphilus strain DH1 is an autotrophic methanogen that was isolated from the wetwood of methane-emitting trees. This species has been of considerable interest for its unusual oxygen tolerance and has been studied as a model organism for more than four decades. Strain DH1 is closely related to other host-associated Methanobrevibacter species from intestinal tracts of animals and the rumen, making this strain an interesting candidate for comparative analysis to identify factors important for colonizing intestinal environments. Here, the genome sequence of M. arboriphilus strain DH1 is reported. The draft genome is composed of 2.445.031 bp with an average GC content of 25.44% and predicted to harbour 1964 protein-encoding genes. Among the predicted genes, there are also more than 50 putative genes for the so-called adhesin-like proteins (ALPs). The presence of ALP-encoding genes in the genome of this non-host-associated methanogen strongly suggests that target surfaces for ALPs other than host tissues also need to be considered as potential interaction partners. The high abundance of ALPs may also indicate that these types of proteins are more characteristic for specific phylogenetic groups of methanogens rather than being indicative for a particular environment the methanogens thrives in.

1. Introduction

Methanogenic archaea (methanogens) comprise a phylogenetically diverse group of microorganisms that can grow in a wide variety of different anoxic environments, such as sediments or the intestinal tracts of animals and humans. The energy metabolism of the two different types of methanogens, those that contain and those that lack cytochromes, has been investigated in detail [1]; however, it remains poorly understood how methanogens respond to some specific environmental stimuli or how they physically interact with their environment. The latter is of particular interest for members of the genus Methanobrevibacter of the order Methanobacteriales. Most Methanobrevibacter species grow by using the same highly conserved hydrogenotrophic methanogenesis pathway, with the genes for this pathway conserved across different Methanobrevibacter species [13]. Yet some species of this genus appear to be more indicative for certain environments than others. For example, previous studies have shown that the methanogen communities in the rumens of cattle and sheep are dominated by two different Methanobrevibacter species, M. gottschalkii and M. ruminantium [4, 5], while microbiota analyses of the human gut revealed that M. smithii is the predominant methanogen in this habitat [68]. The growing number of sequenced Methanobrevibacter genomes allows performing comparative genome analysis in order to identify traits in strains and species that have adapted to a specific environment. It must be noted, however, that all of these genomes have been derived from methanogens that are associated either with the human or animal intestinal tract.

One exception is M. arboriphilus strain DH1 [9], initially named Methanobacterium arbophilicum [10], as this strain does not live in association with an animal or human host. This differentiating characteristic turns M. arboriphilus strain DH1 into ideal candidate for comparative analyses between genomes of host-associated and non-host-associated Methanobrevibacter species. Type strain DH1 was cultivated in 1975 from wetwood of living trees and was considered to be the source of methane emissions from these trees [10]. Since its first isolation, other strains of M. arboriphilus have been isolated from a variety of different environments, such as digested sewage sludge [11], rice paddy soil [12], and more recently the human gut [13]. The gene repertoire of the different M. arboriphilus strains and their adaptations to specific environments as well as the similarity of these different M. arboriphilus strains to each other remains largely unknown.

One remarkable physiological feature of M. arboriphilus strain DH1 is its unusually high oxygen tolerance. This was unexpected, as methanogens and especially most mesophilic members of the cytochrome-lacking methanogen orders were predicted to be particularly oxygen-sensitive due to the lack of cytochrome-oxidase and catalase, two heme proteins. However, physiological characterization of M. cuticularis and M. curvatus from termites and of different M. arboriphilus strains revealed catalase activity in these organisms [14, 15]. In subsequent experiments, it was shown that M. arboriphilus produced a functional heme catalase when the medium was supplemented with hemin [15]. The absence of heme biosynthesis in Methanobrevibacter species and the analysis of the M. arboriphilus catalase gene suggested an acquisition of this gene via horizontal gene transfer [15]. The presence of the catalase gene in some Methanobrevibacter and its absence in others (e.g., M. smithii and M. ruminantium) represent just one example of differential adaptation to a specific environmental condition within the genus.

How Methanobrevibacter species have adapted to specific hosts is not well understood. The analysis of the first genome sequence of a host-associated methanogen, Methanosphaera stadtmanae, revealed some intriguing features in this regard [16]. It was found that the genome of this methanogen encoded almost 40 predicted proteins that were not encoded at all or only in smaller numbers by the genomes of non-host-associated methanogens. These proteins, designated Asn-Thr-rich proteins, are predicted to be longer than the mean protein length, anchored in the membrane via a C- or N-terminal helix and oriented towards the extracellular space, and have an eponymous overrepresentation of asparagine and threonine in their amino acid composition [16]. Meanwhile, several genomes of host-associated Methanobrevibacter strains have been sequenced and their analysis revealed an ubiquitous presence and high abundance of genes encoding Asn-Thr-rich proteins [13, 1722]. This and the remote similarity of these proteins to bacterial homologues led to the annotation of these proteins as adhesin-like proteins (ALPs) [22]. The role for most ALPs remains speculative as there is only limited functional evidence that these proteins act as adhesins and what their targets could be [8]. However, their high abundance in host-associated methanogens suggests a potential role in methanogen-vertebrate host interaction. This hypothesis would be corroborated if ALPs were present only in small numbers or completely absent from non-host-associated Methanobrevibacter strains, such as M. arboriphilus strain DH1.

Here, the genome sequence of Methanobrevibacter arboriphilus strain DH1 is described and compared to the genomes of seven other Methanobrevibacter species (including M. arboriphilus strain ANOR1 (isolated from human feces [13])) and Methanosphaera stadtmanae. The genome analysis and comparisons revealed that M. arboriphilus strain DH1 not only has maintained all genes essential for autotrophic growth but it also shares some genomic features with closely related host-associated methanogens.

2. Materials and Methods

2.1. Cultivation of Microorganism and DNA Extraction

Methanobrevibacter arboriphilus strain DH1 (DSM 1125) was obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ), Braunschweig, Germany. The methanogenic archaeon was cultivated under anaerobic conditions using a medium described by Asakawa et al. [12]. DNA was isolated from the enrichment culture by phenol-chloroform extraction followed by a QIAquick column (Qiagen, Hilden, Germany) purification [23, 24].

2.2. Genome Sequencing

The genome of Methanobrevibacter arboriphilus strain DH1 was sequenced with a combined approach using the 454 GS-FLX Titanium XL system (titanium GS70 chemistry, Roche Life Science, Mannheim, Germany) and the GenomeAnalyser IIx (Illumina, San Diego, CA). Shotgun libraries were prepared according to the manufacturer’s protocols, resulting in 99,511 454 shotgun sequencing reads and 11,827,196 112 bp paired-end Illumina sequencing reads. The Illumina reads were quality trimmed using Trimmomatic version 0.32 [25]. All of the 454 shotgun reads and 2,637,606 of the Illumina reads were used for the initial hybrid de novo assembly with MIRA 3.4 [26] and Newbler 2.8 (Roche Life Science, Mannheim, Germany). The final assembly contained 40 contigs with an average coverage of 92.85. The assembly was validated and the read coverage determined with QualiMap version 2.1 [27]. The quality and the completeness of the draft genome has been validated with CheckM [28].

2.3. Sequence Annotation

The genome data were uploaded to the Integrated Microbial Genomes Expert Review (IMG/ER) platform (https://img.jgi.doe.gov/cgi-bin/er/main.cgi). Coding sequences were predicted and annotated using the automated pipeline of IMG/ER [29]. Briefly, protein-encoding genes were identified with GeneMark [30], and candidate homologue genes of the genomes were computed using BLASTp [31]. Automated annotations of coding sequences were verified and curated by comparing various annotations based on functional resources, such as COG clusters, Pfam, TIGRfam, and gene ontology. The annotated genome sequence of M. arboriphilus strain DH1 (Gs0106968 or Gp0076455) is available in the Genomes Online database (http://www.genomesonline.org/).

2.4. Nucleotide Sequence Accession Number

The annotated genome of Methanobrevibacter arboriphilus strain DH1 has been deposited at DDBJ/EMBL/GenBank under the accession JXMW00000000. The version described in this paper is version JXMW01000000.

2.5. Tree Construction and Taxonomic Assignment

Aligned sequences were selected from RIM-DB [5] and exported in phylip format to construct phylogenetic trees using all available base positions. Maximum likelihood phylogenetic trees based on aligned archaeal 16S rRNA gene sequences were generated using RAxML version 7.0.3 [32]. The parameters “-m GTRGAMMA -# 500 -f a -x 2 -p 2” were used.

2.6. Genome Comparison

Orthologous genes (orthologs) among genome sequences were identified using Proteinortho version 4.26 (default specification: BLAST = BLASTp v2.2.24, value = 1e−10, alg.-conn. = 0.1, coverage = 0.5, percent_identity = 50, adaptive_similarity = 0.95, inc_pairs = 1, inc_singles = 1, selfblast = 1, and unambiguous = 0) [33]. COG categories of the genes were extracted from IMG database entries of M. arboriphilus DH1.

3. Results and Discussion

3.1. General Features

The Methanobrevibacter arboriphilus strain DH1 draft genome has been assembled into 40 contigs (>500 bp), with a N50 of 111,976 bp. Gene characteristics for plasmids were not detected in the assembled contigs. The completeness of the draft genome has been checked by using CheckM [28]. Here, a set of 188 ubiquitous and single copy marker genes was used for validation. Results revealed a 100% completeness of the genome, and in addition, it did not contain any contaminations. The GC content of the draft genome is 25.44%, which is almost identical to the GC content of the recently published draft genome of Methanobrevibacter arboriphilus strain ANOR1 (25.46%) [13], and is in accordance with the overall trend that most Methanobrevibacter and Methanosphaera species genomes tend to have low GC contents between ~24 and 33%, indicating that an early divergent evolution of these two genera from other Methanobacteriales groups may have occurred. Other general features of the genome and a comparison with properties of the Methanobrevibacter arboriphilus strain ANOR1 genome are shown in Table 1 and Figures 1(a) and 1(b).

Table 1
Figure 1: Genomic features of Methanobrevibacter arboriphilus strain DH1 and ANOR1. A circular representation of the M. arboriphilus strain DH1 and comparison with strain ANOR1 is shown in (a). The two outer rings represent both strands of the strain DH1 genome with genes coloured by COGs. The orange/red ring shows genes present in the genome of strain ANOR1 as determined by BLAST. The two inner rings represent GC content and GC skew of strain DH1. The numbers of genes shared by and specific for the two Methanobrevibacter strains are shown in (b). Contigs of the strain DH1 genome were aligned to the genome of strain ANOR1. Ortholog detection was done with the Proteinortho software version 4.26 [33] (BLASTp) with an identity cutoff of 50% and an E value of 1e−10. Visualization was done using Proteinortho results and DNAPlotter [52]. COG categories of the genes were extracted from IMG database entries of M. arboriphilus DH1. Colour code according to E values of the BLASTp analysis performed using Proteinortho 4.26. Grey, 1e−20 to 1; light yellow, 1e−21 to 1e−50; gold, 1e−51 to 1e−90; light orange, 1e−91 to 1e−100; orange, 1e−101 to 1e−120; red, >1e−120.

The genome of strain DH1 also harbours a nonribosomal peptide synthetase (MBBAR_9c00060) and an adjacent phosphopantetheinyl transferase (MBBAR_9c00070). The nonribosomal peptide synthetase (NRPS) shares the highest sequence identity with a Methanobacterium sp. MB1 NRPS gene cluster (49%) and a M. ruminantium NRPS module (43%). NRPS modules are known to produce a wide variety of short-chain peptides, such as antibiotics or siderophores [34]. The function of these proteins in methanogens is currently not understood, and their regulation as well as the potential products of these modules in methanogens remains unclear.

3.2. Genes for Energy Metabolism and Methanogenesis

The gene repertoire required for the energy metabolism of M. arboriphilus strain DH1 is corresponding to that of the other autotrophic Methanobacteriales strain and has been described recently in detail for Methanothermobacter marburgensis [3, 35, 36]. M. arboriphilus strain DH1 is known to grow hydrogenotrophically, utilizing carbon dioxide and hydrogen [10]. The reduction of CO2 to methane proceeds via seven steps. Step one, the reduction of CO2 to formylmethanofuran, is catalysed by formylmethanofuran dehydrogenase (FwdABCDFGH). The genome encodes only the tungsten enzyme, while genes encoding the molybdenum-dependent isoenzyme appear to be absent. The following five steps in methanogenesis are catalysed by formylmethanofuran:H4MPT formyltransferase (Ftr), H4MPT+ cyclohydrolase (Mch), methylene-H4MPT dehydrogenase (Mtd), methylene-H4MPT reductase (Mer), and methyl-H4MPT:coenzyme M methyltransferase (MtrABCDEFGH). The final step is catalysed by methyl-coenzyme M reductase (McrABG). Potential genes encoding isoenzyme II of methyl-coenzyme M reductase (MrtABG) are absent from the draft genome. Similar observations have been made for other Methanobrevibacter species, for example, Methanobrevibacter ruminantium, and it has been speculated that the loss of the Mcr isoenzyme may represent an adaptation to growth at low levels of hydrogen [19, 37]. Gene IDs for genes encoding the enzymes involved in the seven methanogenesis reactions are given in Table S available online at https://doi.org/10.1155/2017/4097425. The majority of the deduced protein sequences share the highest sequence identity with those of the other Methanobacteriales.

The genome also harbours genes encoding a methanol:coenzyme M methyltransferase complex (MtaABC) and the activating protein (MapA), which indicates that the organism may potentially be capable of methylotrophic growth or could utilize methanol to some extent. The subunits of the MtaABC complex share high sequence identity (≥60%) with proteins from Methanobacterium sp. SWAN-1, Methanobacterium lacus, and Methanobrevibacter smithii. So far, no Methanobrevibacter species have been shown to grow on methanol alone or methanol and hydrogen as sole substrates for methanogenesis; however, in a recent study by Samuel et al., an increased expression of the mtaB gene in M. smithii was observed during cocolonization with Bacteroides thetaiotaomicron in mice [22].

3.3. Acetyl-CoA Synthesis

M. arboriphilus strain DH1 has been shown to grow autotrophically with CO2 and H2 in a mineral salts medium that contains vitamins [10]. Carbon assimilation in autotrophic methanogens occurs primarily through carbon monoxide dehydrogenase/acetyl-CoA decarbonylase complex. This complex (MBBAR_1c00380-MBBAR_00390) as well as some of its maturation factors is also encoded by the genome of strain DH1. This complex has thus far not been detected in the genomes of other rumen and intestinal Methanobrevibacter (with few exceptions, such as Methanobrevibacter arboriphilus sp. ANOR1 and some Methanobrevibacter species isolated from the termite hindgut [38]) and Methanosphaera species (M. stadtmanae and M. sp. WGK6 [16, 39]).

3.4. Nitrogen Metabolism

To the authors’ knowledge, it has not been experimentally determined whether strain DH1 is capable of N2 fixation or if this strain is relying on transport systems to import ammonium or amino acids. Genome analysis revealed that both options are potentially available to this strain. The genome harbours genes encoding nitrogenase (MBBAR_1c00660-MBBAR_1c00730), most likely a molybdenum-iron type, which indicates that this organism may be capable of fixing atmospheric nitrogen. Nitrogenase has not been detected in the genomes of closely related intestinal Methanobrevibacter and Methanosphaera species (except for strain ANOR1 and some Methanobrevibacter species isolated from the termite hindgut). In addition to nitrogenase fixation, the genome also contains genes encoding ammonium transporters amt (MBBAR_5c00200, MBBAR_12c00350). Strain DH1 may also be able to import a number of different amino acids/oligopeptides via ABC-type transporters and may derive additional ammonium via deamination of amino acids. The latter would be in agreement with the observation that growth of some methanogens can be greatly improved by supplementation of the medium with amino acids [40].

3.5. Oxygen Detoxification Enzymes in Methanobrevibacter arboriphilus Strain DH1

The oxygen tolerance of M. arboriphilus has been attributed to several oxygen detoxification enzymes, which either protect from oxygen directly or its reactive species. The majority of these enzymes have also been detected in other Methanobacteriales species, but the finding of catalase activity in cell extracts of some Methanobrevibacter species was unexpected. The catalase gene has been cloned from M. arboriphilus strain AZ [15] but had not been known for strain DH1. Here, the catalase gene (MBBAR_24c00090) in strain DH1 was identified and its protein sequence revealed a 99% sequence identity to the enzyme of the AZ strain. The presence of catalase is restricted to only few of the Methanobrevibacter species, and the high protein sequence identity may indicate that the protein was acquired only fairly recently (Figure 2). Strain DH1, like other Methanobacteriales, is not capable of heme biosynthesis and requires therefore hemin supplementation to the medium to produce a functional catalase [15]. Analyses of the genome regions adjacent to the catalase gene did not reveal a transporter gene that could be involved in hemin uptake, and its identification awaits further functional characterization.

Figure 2: Phylogeny of Methanobrevibacter based on the 16S rRNA gene. Grey background indicates the presence of catalase (determined experimentally and/or by presence of the catalase gene). Asterisks behind species names indicate genomes where no apparent homologue of the M. arboriphilus catalase gene was detected by BLAST analysis. The tree was resampled 500 times, and only bootstrap values ≥70% are shown. The dendrogram was rooted with five Crenarchaeota sequences. The scale bar indicates 0.10 inferred nucleotide substitutions per position. M.: Methanobrevibacter.

The strain DH1 genome harbours also genes encoding a number of other enzymes that are involved in the detoxification of oxygen, hydrogen peroxide, and superoxide. Some of these have been identified and characterized in closely related Methanobrevibacter arboriphilus strains, primarily strain AZ. Among these are the genes for rubrerythrin (MBBAR_3c00700), a predicted peroxidase [41], and the recently discovered and characterized F420H2 oxidase (MBBAR_3c00710) [4244]. The proteins have 95% and 91% sequence identity, respectively, with corresponding proteins from strain AZ. The genome also encodes homologues of F420H2 oxidase (MBBAR_6c00420) and another rubrerythrin (MBBAR_6c00090). The latter appears to form a cotranscribed operon with a gene encoding a desulfoferredoxin (MBBAR_6c00080).

3.6. Genes for Adhesin-Like Proteins

One of the main objectives of this study was to determine the presence or absence of adhesin-like proteins. More than 50 gene-encoding ALPs were identified in the genome of M. arboriphilus strain DH1. This number of ALPs in the strain DH1 genome is higher than that in some host-associated Methanobrevibacter genomes. For comparison, M. smithii contains 48, M. sp. AbM4 29 (the currently known lowest number of ALPs in Methanobrevibacter genomes), and M. ruminantium 105 ALPs (the currently known highest number of ALPs in Methanobrevibacter genomes). This finding may indicate that at least some ALPs, if they function as adhesins, may have interaction partners other than host tissues. This hypothesis is corroborated by two findings: first, a recent analysis of a M. ruminantium ALP, Mru_1499, revealed that a domain of these adhesins may be important for the interaction between methanogens and protozoa and bacteria [8]. However, the large and diverse repertoire of methanogen ALPs encoded by some Methanobrevibacter genomes suggests that other interaction partners also need to be considered and that ALP subgroups/domains may have different host/target specificity. Second, smaller numbers of ALPs have also been encoded by genomes of other non-host-associated methanogens, for example, each 12 ALPs have been identified in the genomes of Methanothermobacter marburgensis and M. thermautotrophicus [2, 45, 46].

3.7. Differences between Methanobrevibacter arboriphilus Strains

The only currently available genome sequences of M. arboriphilus strains are those of strain DH1 (isolated from wetwood) and strain ANOR1. A comparative analysis of these strains’ genomes was performed to identify features that are specific for either of the two strains and which may indicate specific adaptations to the environment they were isolated from. Overall, the genomes display a similar genome size and share a large number of genes (Figure 1). Among the shared genes are also some of those that would allow autotrophic growth, such as genes for CODH/ACS-complex and nitrogenase. Strain ANOR1 still awaits a physiological characterization which would help in determining if the enzymes are functional and whether the strain is capable of autotrophic growth.

The lack of genome information for other M. arboriphilus strains currently limits comparative analysis to only two strains. However, more evidence regarding differences between M. arboriphilus strains can be derived from previous physiological and biochemical studies. For example, it has been shown that cell extracts of strain AZ show activity of [Fe]-hydrogenase and the corresponding hmd gene has been cloned and heterologously produced in Escherichia coli [47]. There is no evidence for a hmd gene, hmd isoenzyme genes, or the recently described hmd co-occurring genes in strain DH1 [2]. Gene expression studies in other methanogens have shown that the expression of hmd and the isoenzymes may be regulated by hydrogen and/or also by nickel concentrations, for example, upregulation of hmd under low nickel concentrations [37, 48], but genetic studies have also revealed that hmd may not be required for growth on H2 and CO2 [49]. Depending on the environmental conditions some methanogens live in, these findings could help explain the presence and absence of hmd and co-occurring genes in genomes of some hydrogenotrophic methanogens. In the case of M. arboriphilus, these differences are even present at the strain level. At this stage, it is not possible to explain the reasons for these differences, but it is likely that these phenotypic differences represent specific adaptations to different environmental conditions. A pan-genome approach as recently undertaken for M. smithii [50] may shed light on the gene repertoire of these different strains and how they have adapted to different niches.

3.8. Is M. arboriphilus Strain DH1 Equipped to Survive in an Intestinal Environment?

Some of the genome features, for example, carbon monoxide dehydrogenase/acetyl-CoA decarbonylase complex and nitrogenase and the primary strain description and physiological characteristics, as well as its isolation source, support the finding that strain DH1 is a non-host-associated and autotrophic methanogen. However, it may need to be considered, whether strain DH1 may also be adapted to live in an intestinal environment as suggested by the presence of the high number of ALPs. Analyses of rumen and intestinal methanogens and their genomes have revealed some features, for example, sialic acid synthesis and bile acid hydrolases, which are associated with gut and/or rumen methanogens. The Methanobrevibacter smithii genome has been shown to encode genes predicted to be involved in sialic acid synthesis (neuA and neuB), but genes with only weak similarity to neuA and neuB (MMBAR_10c00110 and MBBAR_10c00100) were detected in strain DH1, and there is no experimental evidence that would support their function in sialic acid synthesis. Similar observations were made for bile acid hydrolases. Functional proof of bile acid hydrolases in Methanosphaera stadtmanae and Methanobrevibacter smithii has been provided, and the corresponding genes for the enzymes in the two genomes have been identified [51]; however, the M. arboriphilus draft genome does not harbour genes encoding closely related homologues of these enzymes. It needs to be taken into account that composition of bile acids and their metabolism may vary between hosts. Knowing the potential host may therefore be a prerequisite to determine the activity of such an enzyme in strain DH1.

It may also be necessary to consider intestinal environments other than the vertebrate intestinal tract as a habitat for strain DH1. The 16S rRNA gene of the strain shares high sequence identity with isolates from the termite hindgut (Figure 2), and also, some characteristic features, such as catalase, have been identified in M. arboriphilus and termite hindgut Methanobrevibacter strains [38]. Genome comparisons indicate that strain DH1 shares more genes with each of the Methanobrevibacter strains from the termite hindgut (1168–1293) than with species from the animal or human intestinal tract (1116–1151, excluding M. arboriphilus strain ANOR1). Some of the genes shared by strain DH1 and the termite hindgut Methanobrevibacter strains include genes encoding nitrogenase (present in all three termite hindgut Methanobrevibacter strains) and acetyl-CO-synthase/carbon monoxide dehydrogenase complex (present in M. cuticularis and M. filiformis) [38]. These findings suggest that it may be possible that M. arboriphilus strain DH1 could also be originating from an arthropod-associated microbiome. Alternatively, it could also indicate that Methanobrevibacter strains associated with the termite hindgut have conserved more genes for autotrophic growth in their genomes than the predominant Methanobrevibacter species from vertebrate intestinal tracts.

4. Conclusion

The genome of M. arboriphilus strain DH1 provides the first genome sequence of an autotrophic Methanobrevibacter species that is not associated with the gastrointestinal tract or rumen and serves as an important reference sequence for comparative genomics. The high abundance of adhesin-like proteins in this Methanobrevibacter genome was unexpected and raises further questions about their function and interaction partners. These results reveal that ALPs may be a common feature of some methanogen clades, but they may not be involved in the interaction between the methanogen and the surface of host tissues to the extent as previously assumed.

Conflicts of Interest

The authors declare that they have no conflicts of interest.

Acknowledgments

The authors thank Rolf Thauer, MPI Marburg, and Peter Janssen, AgResearch Ltd., for the support and comments; Frauke-Dorothee Meyer and Kathleen Gollnow for the technical support; and Jonathan Humphreys for the proofreading of the manuscript. This work was funded by the Temasek Life Sciences Laboratory.

References

  1. R. K. Thauer, A. K. Kaster, H. Seedorf, W. Buckel, and R. Hedderich, “Methanogenic archaea: ecologically relevant differences in energy conservation,” Nature Reviews Microbiology, vol. 6, no. 8, pp. 579–591, 2008. View at Publisher · View at Google Scholar · View at Scopus
  2. A. K. Kaster, M. Goenrich, H. Seedorf et al., “More than 200 genes required for methane formation from H2 and CO2 and energy conservation are present in Methanothermobacter marburgensis and Methanothermobacter thermautotrophicus,” Archaea, vol. 2011, 23 pages, 2011. View at Publisher · View at Google Scholar · View at Scopus
  3. R. K. Thauer, “Biochemistry of methanogenesis: a tribute to Marjory Stephenson: 1998 Marjory Stephenson prize lecture,” Microbiology, vol. 144, no. 9, pp. 2377–2406, 1998. View at Publisher · View at Google Scholar
  4. P. H. Janssen and M. Kirs, “Structure of the archaeal community of the rumen,” Applied and Environmental Microbiology, vol. 74, no. 12, pp. 3619–3625, 2008. View at Publisher · View at Google Scholar · View at Scopus
  5. H. Seedorf, S. Kittelmann, G. Henderson, and P. H. Janssen, “RIM-DB: a taxonomic framework for community structure analysis of methanogenic archaea from the rumen and other intestinal environments,” PeerJ, vol. 2, no. e494, 2014. View at Publisher · View at Google Scholar · View at Scopus
  6. B. Dridi, M. Henry, A. El Khechine, D. Raoult, and M. Drancourt, “High prevalence of Methanobrevibacter smithii and Methanosphaera stadtmanae detected in the human gut using an improved DNA detection protocol,” PloS One, vol. 4, no. 9, article e7063, 2009. View at Publisher · View at Google Scholar · View at Scopus
  7. T. L. Miller and M. J. Wolin, “Enumeration of Methanobrevibacter smithii in human feces,” Archives of Microbiology, vol. 131, no. 1, pp. 14–18, 1982. View at Publisher · View at Google Scholar · View at Scopus
  8. F. Ng, S. Kittelmann, M. L. Patchett et al., “An adhesin from hydrogen-utilizing rumen methanogen Methanobrevibacter ruminantium M1 binds a broad range of hydrogen-producing microorganisms,” Environmental Microbiology, vol. 18, no. 9, pp. 3010–3021, 2016. View at Publisher · View at Google Scholar · View at Scopus
  9. T. L. Miller, “Methanobrevibacter,” in Bergey's Manual of Systematics of Archaea and Bacteria, pp. 218–226, Springer Verlag, New York, 2001. View at Publisher · View at Google Scholar
  10. J. Zeikus and D. L. Henning, “Methanobacterium arbophilicum sp. nov. an obligate anaerobe isolated from wetwood of living trees,” Antonie van Leeuwenhoek, vol. 41, no. 4, pp. 543–552, 1975. View at Publisher · View at Google Scholar · View at Scopus
  11. A. J. Zehnder and K. Wuhrmann, “Physiology of a Methanobacterium strain AZ,” Archives of Microbiology, vol. 111, no. 3, pp. 199–205, 1977. View at Publisher · View at Google Scholar · View at Scopus
  12. S. Asakawa, H. Morii, M. Akagawa-Matsuhita, Y. Koga, and K. Hayano, “Characterization of Methanobrevibacter arboriphilicus SA isolated from a paddy field soil and DNA-DNA hybridization among M. arboriphilicus strains,” International Journal of Systematic and Evolutionary Microbiology, vol. 43, no. 4, pp. 683–686, 1993. View at Google Scholar
  13. S. Khelaifia, M. Garibal, C. Robert, D. Raoult, and M. Drancourt, “Draft genome sequence of a human-associated isolate of Methanobrevibacter arboriphilicus, the lowest-G+C-content archaeon,” Genome Announcements, vol. 2, no. 1, article e01181-13, 2014. View at Publisher · View at Google Scholar
  14. J. R. Leadbetter and J. A. Breznak, “Physiological ecology of Methanobrevibacter cuticularis sp. nov. and Methanobrevibacter curvatus sp. nov., isolated from the hindgut of the termite Reticulitermes flavipes,” Applied and Environmental Microbiology, vol. 62, no. 10, pp. 3620–3631, 1996. View at Google Scholar
  15. S. Shima, M. Sordel-Klippert, A. Brioukhanov, A. Netrusov, D. Linder, and R. K. Thauer, “Characterization of a heme-dependent catalase from Methanobrevibacter arboriphilus,” Applied and Environmental Microbiology, vol. 67, no. 7, pp. 3041–3045, 2001. View at Publisher · View at Google Scholar · View at Scopus
  16. W. F. Fricke, H. Seedorf, A. Henne et al., “The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis,” Journal of Bacteriology, vol. 188, no. 2, pp. 642–658, 2006. View at Publisher · View at Google Scholar · View at Scopus
  17. W. J. Kelly, D. Li, S. C. Lambie et al., “Draft genome sequence of the rumen methanogen Methanobrevibacter olleyae YLM1,” Genome Announcements, vol. 4, no. 2, article e00232-16, 2016. View at Publisher · View at Google Scholar
  18. S. Khelaifia, M. Garibal, C. Robert, D. Raoult, and M. Drancourt, “Draft genome sequencing of Methanobrevibacter oralis strain JMR01, isolated from the human intestinal microbiota,” Genome Announcements, vol. 2, no. 1, article e00073-14, 2014. View at Publisher · View at Google Scholar
  19. S. C. Leahy, W. J. Kelly, E. Altermann et al., “The genome sequence of the rumen methanogen Methanobrevibacter ruminantium reveals new possibilities for controlling ruminant methane emissions,” PloS One, vol. 5, no. 1, article e8926, 2010. View at Publisher · View at Google Scholar
  20. S. C. Leahy, W. J. Kelly, D. Li et al., “The complete genome sequence of Methanobrevibacter sp. AbM4,” Standards in Genomic Sciences, vol. 8, no. 2, pp. 215–227, 2013. View at Publisher · View at Google Scholar · View at Scopus
  21. J.-H. Lee, M. S. Rhee, S. Kumar et al., “Genome sequence of Methanobrevibacter sp. strain JH1, isolated from rumen of Korean native cattle,” Genome Announcements, vol. 1, no. 1, article e00002-13, 2013. View at Publisher · View at Google Scholar · View at Scopus
  22. B. S. Samuel, E. E. Hansen, J. K. Manchester et al., “Genomic and metabolic adaptations of Methanobrevibacter smithii to the human gut,” Proceedings of the National Academy of Sciences, vol. 104, no. 25, pp. 10643–10648, 2007. View at Publisher · View at Google Scholar · View at Scopus
  23. S. Kittelmann, C. S. Pinares-Patiño, H. Seedorf et al., “Two different bacterial community types are linked with the low-methane emission trait in sheep,” PloS One, vol. 9, no. 7, article e103171, 2014. View at Publisher · View at Google Scholar · View at Scopus
  24. A. Rius, S. Kittelmann, K. Macdonald, G. Waghorn, P. Janssen, and E. Sikkema, “Nitrogen metabolism and rumen microbial enumeration in lactating cows with divergent residual feed intake fed high-digestibility pasture,” Journal of Dairy Science, vol. 95, no. 9, pp. 5024–5034, 2012. View at Publisher · View at Google Scholar · View at Scopus
  25. A. M. Bolger, M. Lohse, and B. Usadel, “Trimmomatic: a flexible trimmer for Illumina sequence data,” Bioinformatics, vol. 30, no. 15, pp. 2114–2120, 2014. View at Publisher · View at Google Scholar · View at Scopus
  26. B. Chevreux, T. Wetter, and S. Suhai, “Genome sequence assembly using trace signals and additional sequence information,” German Conference on Bioinformatics, vol. 99, pp. 45–56, 1999. View at Google Scholar
  27. F. García-Alcalde, K. Okonechnikov, J. Carbonell et al., “Qualimap: evaluating next-generation sequencing alignment data,” Bioinformatics, vol. 28, no. 20, pp. 2678-2679, 2012. View at Publisher · View at Google Scholar · View at Scopus
  28. D. H. Parks, M. Imelfort, C. T. Skennerton, P. Hugenholtz, and G. W. Tyson, “CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes,” Genome Research, vol. 25, no. 7, pp. 1043–1055, 2015. View at Publisher · View at Google Scholar · View at Scopus
  29. V. M. Markowitz, K. Mavromatis, N. N. Ivanova, I.-M. A. Chen, K. Chu, and N. C. Kyrpides, “IMG ER: a system for microbial genome annotation expert review and curation,” Bioinformatics, vol. 25, pp. 2271–2278, 2009. View at Publisher · View at Google Scholar · View at Scopus
  30. J. Besemer and M. Borodovsky, “GeneMark: web software for gene finding in prokaryotes, eukaryotes and viruses,” Nucleic Acids Research, vol. 33, Supplement 2, pp. W451–W454, 2005. View at Publisher · View at Google Scholar · View at Scopus
  31. S. F. Altschul, T. L. Madden, A. A. Schäffer et al., “Gapped BLAST and PSI-BLAST: a new generation of protein database search programs,” Nucleic Acids Research, vol. 25, no. 17, pp. 3389–3402, 1997. View at Publisher · View at Google Scholar · View at Scopus
  32. A. Stamatakis, “RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models,” Bioinformatics, vol. 22, no. 21, pp. 2688–2690, 2006. View at Publisher · View at Google Scholar · View at Scopus
  33. M. Lechner, S. Findeiß, L. Steiner, M. Marz, P. F. Stadler, and S. J. Prohaska, “Proteinortho: detection of (co-) orthologs in large-scale analysis,” BMC Bioinformatics, vol. 12, no. 1, p. 124, 2011. View at Publisher · View at Google Scholar · View at Scopus
  34. D. Schwarzer, R. Finking, and M. A. Marahiel, “Nonribosomal peptides: from genes to products,” Natural Product Reports, vol. 20, no. 3, pp. 275–287, 2003. View at Publisher · View at Google Scholar · View at Scopus
  35. A.-K. Kaster, J. Moll, K. Parey, and R. K. Thauer, “Coupling of ferredoxin and heterodisulfide reduction via electron bifurcation in hydrogenotrophic methanogenic archaea,” Proceedings of the National Academy of Sciences, vol. 108, no. 7, pp. 2981–2986, 2011. View at Publisher · View at Google Scholar · View at Scopus
  36. R. K. Thauer, “The Wolfe cycle comes full circle,” Proceedings of the National Academy of Sciences, vol. 109, no. 39, pp. 15084-15085, 2012. View at Publisher · View at Google Scholar · View at Scopus
  37. N. Reeve, J. Nölling, R. M. Morgan, and D. R. Smith, “Methanogenesis: genes, genomes, and who’s on first?” Journal of Bacteriology, vol. 179, no. 19, p. 5975, 1997. View at Publisher · View at Google Scholar
  38. A. Poehlein and H. Seedorf, “Draft genome sequences of Methanobrevibacter curvatus DSM11111, Methanobrevibacter cuticularis DSM11139, Methanobrevibacter filiformis DSM11501, and Methanobrevibacter oralis DSM7256,” Genome Announcements, vol. 4, no. 3, article e00617-16, 2016. View at Publisher · View at Google Scholar
  39. E. C. Hoedt, P. Ó. Cuív, P. N. Evans et al., “Differences down-under: alcohol-fueled methanogenesis by archaea present in Australian macropodids,” The ISME Journal, vol. 10, no. 10, pp. 2376–2388, 2016. View at Publisher · View at Google Scholar · View at Scopus
  40. M. Bryant, S. Tzeng, I. Robinson, and A. Joyner Jr, Nutrient Requirements of Methanogenic Bacteria, ACS Publications, United States, 1971.
  41. P. S. Alban and N. R. Krieg, “A hydrogen peroxide resistant mutant of Spirillum volutans has NADH peroxidase activity but no increased oxygen tolerance,” Canadian Journal of Microbiology, vol. 44, no. 1, pp. 87–91, 1998. View at Publisher · View at Google Scholar
  42. H. Seedorf, A. Dreisbach, R. Hedderich, S. Shima, and R. K. Thauer, “F420H2 oxidase (FprA) from Methanobrevibacter arboriphilus, a coenzyme F420-dependent enzyme involved in O2 detoxification,” Archives of Microbiology, vol. 182, no. 2-3, pp. 126–137, 2004. View at Publisher · View at Google Scholar · View at Scopus
  43. H. Seedorf, C. H. Hagemeier, S. Shima, R. K. Thauer, E. Warkentin, and U. Ermler, “Structure of coenzyme F420H2 oxidase (FprA), a di-iron flavoprotein from methanogenic archaea catalyzing the reduction of O2 to H2O,” FEBS Journal, vol. 274, no. 6, pp. 1588–1599, 2007. View at Publisher · View at Google Scholar · View at Scopus
  44. H. Seedorf, J. Kahnt, A. J. Pierik, and R. K. Thauer, “Si-face stereospecificity at C5 of coenzyme F420 for F420H2 oxidase from methanogenic archaea as determined by mass spectrometry,” FEBS Journal, vol. 272, no. 20, pp. 5337–5342, 2005. View at Publisher · View at Google Scholar · View at Scopus
  45. H. Liesegang, A. K. Kaster, A. Wiezer et al., “Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism,” Journal of Bacteriology, vol. 192, no. 21, pp. 5850-5851, 2010. View at Publisher · View at Google Scholar · View at Scopus
  46. D. R. Smith, L. A. Doucette-Stamm, C. Deloughery et al., “Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics,” Journal of Bacteriology, vol. 179, no. 22, pp. 7135–7155, 1997. View at Publisher · View at Google Scholar
  47. O. Pilak, Kristallstruktur von [Fe]-Hydrogenase Hmd, Universitätsbibliothek, Marburg, 2007.
  48. C. Afting, E. Kremmer, C. Brucker, A. Hochheimer, and R. K. Thauer, “Regulation of the synthesis of H 2-forming methylenetetrahydromethanopterin dehydrogenase (Hmd) and of HmdII and HmdIII in Methanothermobacter marburgensis,” Archives of Microbiology, vol. 174, no. 4, pp. 225–232, 2000. View at Publisher · View at Google Scholar · View at Scopus
  49. E. L. Hendrickson and J. A. Leigh, “Roles of coenzyme F420-reducing hydrogenases and hydrogen-and F420-dependent methylenetetrahydromethanopterin dehydrogenases in reduction of F420 and production of hydrogen during methanogenesis,” Journal of Bacteriology, vol. 190, no. 14, pp. 4818–4821, 2008. View at Publisher · View at Google Scholar · View at Scopus
  50. E. E. Hansen, C. A. Lozupone, F. E. Rey et al., “Pan-genome of the dominant human gut-associated archaeon, Methanobrevibacter smithii, studied in twins,” Proceedings of the National Academy of Sciences, vol. 108, Supplement 1, pp. 4599–4606, 2011. View at Publisher · View at Google Scholar · View at Scopus
  51. B. V. Jones, M. Begley, C. Hill, C. G. Gahan, and J. R. Marchesi, “Functional and comparative metagenomic analysis of bile salt hydrolase activity in the human gut microbiome,” Proceedings of the National Academy of Sciences, vol. 105, no. 36, pp. 13580–13585, 2008. View at Publisher · View at Google Scholar · View at Scopus
  52. T. Carver, N. Thomson, A. Bleasby, M. Berriman, and J. Parkhill, “DNAPlotter: circular and linear interactive genome visualization,” Bioinformatics, vol. 25, no. 1, pp. 119-120, 2009. View at Publisher · View at Google Scholar · View at Scopus