Research Article

Construction of Expression Shuttle Vectors for the Haloarchaeon Natrinema sp. J7 Based on Its Chromosomal Origins of Replication

Table 1

Strains and plasmids used in this study.

StrainDescriptionSource

Natrinema sp. J7-1With SNJ1 proviral genome pHH205, cannot be infected by SNJ1[28, 34]
Natrinema sp. J7-FΔpyrF, can be infected by SNJ1Yuping Huang, Wuhan University, Wuhan, China
Escherichia coli DH5αSupE44 ΔlacU169(80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1CCTCC
Escherichia coli JM110dam dcm supE44 hsdR17 thi leu rpsL lacY galK galT ara tonA thr tsx Δ(lac-proAB) F′(traD36 proAB+ lacZ ΔM15)CCTCC
Plasmid
pNBK-FAmpR, MevR, used to clone -pyrF resistance fragmentYuping Huang, Wuhan University, Wuhan, China
pUC-MpUC19 with SnaBI, MfeI, AgeI inserted between BamHI and XbalOur lab
pUC-M-pyrFpUC-M with the insertion of 0.95-kb BamHI-SnaBI -pyrF resistance fragmentThis study
pFJ1pUC-M-pyrF with the insertion of the 2.8-kb SnaBI-MfeI predicted chromosomal ARS-1This study
pFJ2pUC-M-pyrF with the insertion of the 3.3-kb SnaBI-MfeI predicted chromosomal ARS-2This study
pFJ3pUC-M-pyrF with the insertion of the 3.2-kb SnaBI-MfeI predicted chromosomal r ARS-3This study
pFJ4pUC-M-pyrF with the insertion of the 2.2-kb SnaBI-MfeI predicted chromosomal ARS-4This study
pFJ5pUC-M-pyrF with the insertion of the 2.3-kb SnaBI-MfeI predicted chromosomal ARS-5This study
pFJ6pUC-M-pyrF with the insertion of the 2.0-kb SnaBI-MfeI predicted chromosomal ARS-6This study
pFJ7pUC-M-pyrF with the insertion of the 1.1-kb SnaBI-MfeI predicted chromosomal ARS-7This study
pFJ1-Apro-amyHpFJ1 with the insertion of 1.5-kb MfeI-SphI Apro-amyH fragment containing AflII, SpeI, NotI, NheI, and NsiIThis study
pFJ1-MpFJ1-Apro-amyH digested with NotI and then ligated by itselfThis study
pFJ4-Apro-amyHpFJ4 with the insertion of 1.5-kb MfeI-SphI Apro-amyH fragment containing AflII, SpeI, NotI, NheI, and NsiIThis study
pFJ4-MpFJ4-Apro-amyH digested with NotI and then ligated by itselfThis study
pFJ6-Apro-amyHpFJ6 with the insertion of 1.5-kb MfeI-SphI Apro-amyH fragment containing AflII, SpeI, NotI, NheI, and NsiIThis study
pFJ6-MpFJ6-Apro-amyH digested with NotI and then ligated by itselfThis study
pFJ1-M-pro3916pFJ1-M with the insertion of 200-bp NsiI-SphI pro3916This study
pFJ4-M-pro3916pFJ4-M with the insertion of 200-bp NsiI-SphI pro3916This study
pFJ6-M-pro3916pFJ6-M with the insertion of 200-bp NsiI-SphI pro3916This study