Expression, Purification, and Characterization of (R)-Sulfolactate Dehydrogenase (ComC) from the Rumen Methanogen Methanobrevibacter millerae SM9

Table 1

Kinetic parameters for ComC, for sulfopyruvate, and for NADH. Standard assay conditions in the reduction reaction were used; 400 mM KCl, pH 6.5, and 37°C. Assays were carried out in triplicate. One unit of activity (U) is defined as the conversion of one μmol of NADH to NAD⁺ per minute under standard assay conditions.

	KM (μM)	Vmax (U·mg^−1)	kcat (s^−1)
Sulfopyruvate	196 ± 71	93.9 ± 14.5	62.8 ± 9.7
NADH	55.1 ± 7.6	73.1 ± 3.2	48.9 ± 2.2