An Acidic Exopolysaccharide from Haloarcula hispanica ATCC33960 and Two Genes Responsible for Its Synthesis

Table 1

Oligonucleotides used in this study.

Primers	Sequences(5′ → 3′)	Restriction site
p1	CCCAAGCTTTATGGCCGAGAACATCCTCG	Hind III
p2	CCGTCGATTGAAACGGTTTGGGAATTAGTAAATTAG
p3	CTAATTTACTAATTCCCAAACCGTTTCAATCGACGG
p4	CGGGGTACCCGCATACCTCTTGGTATAG	Kpn І
p5	GGAATTCCATATGGATATCCTCCACACGCC	Nde І
p6	GGGGTACCCTAAACTAAGTCTTCATGTACC	Kpn І
p7	AACCGTTTCAATCGACGGTATATCCTGATTATTC
p8	ATCTCACAACATCTGTTGATTCTGGCATTCACAAG
p9	CCCAAGCTTCACTACATCATCCAAACTTC	Hind III
p10	TTCTCTTTTTCGTTGACCAAAAGCGATGTTTGTCATTC
p11	ATGACAAACATCGCTTTTGGTCAACGAAAAAGAGAACG
p12	CGGGGTACCTCACGAGACGATCATGG	Kpn І
p13	CGCGGATCCATTTTCCAGGGATCTTTCAAATG	BamH І
p14	GGGGTACCGCTTTGGGGAGATCCGTGTAACTC	Kpn І
p15	GGTCAACGAAAAAGAGAACGTGGCAAGAAGTG
p16	CCCGGAGGTAATGCACCAGCGATGGCTCGAAAC
p17	ATGGATATCCTCCACACGCC
p18	CATGTACCTCGTTATGATCG
p19	GTGAGTAATGTTCTGTATCC
p20	GCCCTGTATGCTTCCAGTGC
p21	ATGGCAAATACACCGGTATCAG
p22	TACTACACTGCCACCGGGTTC
p23	ATGACAGACGCCGCGTCCCTC
p24	CAGGTACACCGAGTTGCCGA