Research Article

A Flexible System for Cultivation of Methanococcus and Other Formate-Utilizing Methanogens

Figure 2

Growth of M. maripaludis S2 with 200 mM formate and 100 mM of Tris, glycine, and glycylglycine buffers. Two kinds of serum bottle stoppers were used. Blue stoppers are thick butyl rubber stoppers (Bellco Glass Inc., Vineland, NJ, cat. number: 2048-11800, 704.82 USD/1000). They are commonly used for H2/CO2 medium. Butyl rubber gray stoppers (Wheaton Science Products, cat. number: W224100-202, 174.2 USD/1000) were also tested for their durability during M. maripaludis cultivation.