A Flexible System for Cultivation of Methanococcus and Other Formate-Utilizing Methanogens

Figure 6

Gassing manifold for exchanging headspace of culture tubes. (A) Vacuum-pressure gauge (Swagelok, cat. number: PGI-63C-OC60-LAQX); (B) three-way valve with Swagelok fittings (Swagelok, cat. number: B-43XS4) for connection to sources of O₂-free gas and vacuum; (C) Swagelok tube fitting, union cross, 1/4 in. (Swagelok, cat. number: B-400-4); (D) brass Swagelok tube fitting, reducer, 1/8 in. × 1/4 in. (Swagelok, cat. number: B-200-R-4); (E) thin bore polyethylene tubing, 1/8 in. (Freelin-Wade Co., cat. number: 1A-109-01); (F) PFA Swagelok tube fitting, reducing union, 1/4 × 1/8 in. (Swagelok, cat. number: PFA-420-6-2); (G) 1 cc plastic syringe barrel (BD, cat. number: 309659; cut off grip on the end of the barrel to allow insertion into the union); and (H) disposable needle (BD, 22 G × 1 in., cat. number: 305155).