A Flexible System for Cultivation of Methanococcus and Other Formate-Utilizing Methanogens

Figure 7

Scheme of picking colonies from agar bottles at the gassing station. (a) A 70 mL serum bottle plated with M. maripaludis S2 forms colonies after 3 days. After opening, a sterile N₂ gassing cannula was introduced (a) to prevent air from entering the bottle and to maintain anaerobiosis. A 1 mL syringe with a 22 G × 1-inch needle was used for transferring the colony (b). To pick the colony more accurately, the needle was bent about 45 degrees by folding the end of the needle against the needle cap. (b) Enlarged image of the 1 mL syringe with the bent needle.

(a)

(b)