Transfection Studies with Colloidal Systems Containing Highly Purified Bipolar Tetraether Lipids from Sulfolobus acidocaldarius

Table 2

Composition and physicochemical properties of polyplexes and lipoplexes.

Formulation (mol : mol) [N/P ratio]	Particle size ± SD [nm]	AFM diameter [nm]	Zeta potential [mV]
25 kDa bPEI	60.2 ± 10.2	79.5	+31.2
DOTAP		257.1	+38.9
hGDNT/DOTAP (5 : 95) [2.5]		317.4	+41.0
PLFE/DOTAP (5 : 95) [2.5]	214.5 ± 10.2	210.7	+42.7
hGDNT/CH/DOTAP (5 : 45 : 50)	450.1 ± 4.5	473.5	+50.1
PLFE/CH/DOTAP (5 : 45 : 50)	372.3 ± 15.0	441.3	+51.6
hGDNT/PC/CH/DOTAP (5 : 30 : 35 : 30)	231.8 ± 35.0	398.4	−48.6