Transcriptional activity of S. solfataricus
whole cell extracts. In vitro transcription reactions were performed using S. solfataricus
S30 fractions with (α
P) UTP in different experimental conditions as described in Material and Methods
and Table 1
. Reaction A was incubated at 60°C while reaction B was incubated at 70°C. Total RNA was extracted from the reaction mixes, and an aliquot of the samples was treated with RNase A at 37°C for 30 min. The products of in vitro transcription were subjected to nondenaturing polyacrylamide gel electrophoresis, and those incorporating (α
P) UTP were visualized by autoradiography.