analysi genom sequenc data from methanogen archaeon methanosarcina mazei gö1 reveal exist two open read frame encod protontransloc pyrophosphatas ppase these open read frame link 750bp intergen region contain tcrich stretch transcrib opposit direct the correspond polypeptid refer mvp1 mvp2 consist 671 676 amino acid respect both enzym repres extrem hydrophob integr membran protein 15 predict transmembran segment overal amino acid sequenc similar 501 multipl sequenc align reveal mvp1 close relat eukaryot ppase wherea mvp2 show highest homolog bacteri ppase northern blot experi rna from methanolgrown cell harvest midlog growth phase indic onli mvp2 produc under condit analysi wash membran show mvp2 had specif activ 034 protein 1 proton transloc experi invert membran vesicl prepar from methanolgrown cell show hydrolysi 1 mol pyrophosph coupl transloc about 1 mol proton across cytoplasm membran appropriat condit mvp 1 express could determin yet the pyrophosphatas mazei gö1 repres first exampl enzym class methanogen archaea may part energyconserv system abbreviat dccd dicyclohexylcarbodiimid ppase inorgan pyrophosphatas ppi inorgan pyrophosph proton motiv forc

Sebastian Bäumer

Sabine Lentes

Gerhard Gottschalk

Uwe Deppenmeier

Identification and analysis of proton-translocating pyrophosphatases in the methanogenic archaeon Methanosarcina mazei

About this journal

Archaea publishes original research articles as well as review articles dealing with all aspects of archaea, providing a unique venue for exchanging information about these extraordinary prokaryotes.

Journal profile

Meet the editorial board

Archaea maintains an Editorial Board of practicing researchers from around the world, to ensure manuscripts are handled by editors who are experts in the field of study.

Editor spotlight

Submit your proposal

Do you think there is an emerging area of research that really needs to be highlighted? Or an existing research area that has been overlooked or would benefit from deeper investigation? Raise the profile of a research area by leading a Special Issue.

Archaea

Table of Contents

Table of Contents: 2002

Identification and analysis of proton-translocating pyrophosphatases in the methanogenic archaeon Methanosarcina mazei