Research Article

Characterization and Quenching of Autofluorescence in Piglet Testis Tissue and Cells

Figure 1

Autofluorescence observed in whole-mount seminiferous cords by epifluorescent and confocal laser scanning microscopes. Seminiferous cords were dissociated from 1-wk-old piglet testes and examined under an epifluorescent microscope (a–c) equipped with filters of A (a), I3 (b), and N21 (c). Confocal laser scanning microscopy with excitation laser of 405 nm, brightfield (d), or brightfield overlaid with acquired signal from spectrums of blue (440–490 nm, e), green (495–570 nm, f), yellow (575–620 nm, g), and red (625–780 nm, h). Scale bars, 100 μm.
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