Characterization and Quenching of Autofluorescence in Piglet Testis Tissue and Cells

Figure 3

Autofluorescence in testis tissue sections from pigs of different ages observed using a confocal laser scanning microscope. Testis tissues from 1-wk-old (a–d), 2-month-old (e–h), and mature (i–l) pigs were fixed, sectioned, and examined for intrinsic fluorescence under a confocal laser scanning microscope with excitation by a 405 nm laser and acquisition of signal from spectrums of blue (440–490 nm; a, e, i), green (495–570 nm; b, f, g), yellow (575–620 nm; c, g, k), and red (625–780 nm; d, h, l) with brightfield overlay. Scale bars, 100 μm.

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