Subcellular Microanatomy by 3D Deconvolution Brightfield Microscopy: Method and Analysis Using Human Chromatin in the Interphase Nucleus
Figure 2
Chromatin texture measurement on all 12 cells used in this study by the third moment of Hu. The value of the third moment calculation is shown on the ordinate versus the cell measured on the abscissa (the 6 plasma cells followed by the 6 hepatocytes). The graph (a) shows the measurements as made on a 2D slice through the centre of each nucleus. The graph (b) shows the same calculation but performed on the whole 3D reconstructed nucleus. Clearly the measurement made on the 3D nucleus gives greater uniformity of the values between cells of similar type and greater distinction between different types of cell.
