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Anatomy Research International
Volume 2012 (2012), Article ID 848707, 7 pages
http://dx.doi.org/10.1155/2012/848707
Research Article

Subcellular Microanatomy by 3D Deconvolution Brightfield Microscopy: Method and Analysis Using Human Chromatin in the Interphase Nucleus

Department of Histopathology, Northwick Park Hospital, Watford Road, London HA1 3UJ, UK

Received 23 July 2011; Accepted 19 October 2011

Academic Editor: Mustafa F. Sargon

Copyright © 2012 Paul Joseph Tadrous. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Movie 1. This is a “fly-into” sequences of the 3D structure of a typical plasma cell nucleus. The grey-levels have been inverted to aid visual 3D depth cues but this is brightfield imagery. Note the prominent peripheral clumps of chromatin without a central clump. The internal chromatin connections are relatively sparse and coarse compared to those of a hepatocyte (see movie 2). Also note the fine connections between peripheral clumps which are not obvious from an external inspection as the dominant features of the large clumps throughout the nucleus superimpose on an external view. Thus the ability to fly “into” the nucleus and look at these clumps from the inside out reveals morphological detail not otherwise easily appreciable and is one of the advantages of the methods presented in this study.

Supplementary Movie 2. This is a “fly-into” sequences of the 3D structure of a typical hepatocyte nucleus. The grey-levels have been inverted to aid visual 3D depth cues but this is brightfield imagery. Note how the internal chromatin in the hepatocyte is characterised by more complex multiply-branched internal connections and is more dispersed in linear strands in contrast to the plasma cell.

  1. Supplementary Movie 1
  2. Supplementary Movie 2