Expression and Function of the Chemokine, CXCL13, and Its Receptor, CXCR5, in Aids-Associated Non-Hodgkin's Lymphoma
Figure 3
AIDS-NHL cell lines express CXCR5, as shown by flow cytometry. The AIDS-BL cell line, 2F7 (a), and the AIDS-DLBCL cell line, R (b), were stained for CXCR5 expression using an indirect staining protocol, as noted in Section 2. First, cells were stained with a rat anti-CXCR5 antibody (dotted lines). As a control, some cells were stained with a rat isotype control antibody (solid lines). Cells were then stained with a PE-conjugated goat anti-rat IgG secondary antibody, and examined by flow cytometry. During the flow cytometry acquisition stage, at least 5,000 cells/events were acquired per tube/condition. During analysis, dead cells were excluded using forward- and side-scatter.
