Expression and Function of the Chemokine, CXCL13, and Its Receptor, CXCR5, in Aids-Associated Non-Hodgkin's Lymphoma

Figure 4

AIDS-NHL cell lines demonstrate chemotaxis towards CXCL13 in vitro. As noted in Section 2, cells of the AIDS-NHL cell lines, 2F7 (AIDS-BL) or R (AIDS-DLBCL), were first prelabeled with Calcein-AM, and then placed on top of 96-well chemotaxis chambers and permitted to migrate for 2 hours in response to CXCL13 or media alone placed in the lower wells. To create a standard curve, preset numbers of cells were added to some wells. Fluorescence in the bottom well was read in a plate reader, and this data was converted to number of cells migrated using the standard curve. As noted in Section 3, 100 ng/mL of CXCL13 was used for the 2F7 cell line, and 50 ng/mL was used for the R cell line. Each experiment was replicated at least 3 times, and, in each experiment, 6–12 replicate wells were used for each experimental condition. The figure shows data from a representative experiment for each cell line. Results are shown as average percent of cells migrating compared to the media only controls (which are set at 100%). Solid bars show results for media alone controls; hatched bars show results after addition of CXCL13. Bars represent the SEM.