Analytical Performances of Human Immunodeficiency Virus Type 1 RNA-Based Amplix® Real-Time PCR Platform for HIV-1 RNA Quantification

Table 2

Repeatability and reproducibility of the HIV-1 RNA-based Amplix real-time PCR assay.

Panel	Target HIV-1 RNA (log copies/mL)	Number of determinations	Mean (SD) (log copies/mL) of HIV-1 RNA measured	Coefficient of variation (%)
Repeatability
Third WHO standard	5.04	3	5.2 (0.3)	5.7
Low-positive pool	3.17	30	3.2 (0.2)	7.7
High-positive pool	6.33	30	6.5 (0.3)	4.6
Reproducibility
Third WHO standard	5.04	3	5.3 (0.4)	7.5
Low-positive pool	3.17	30	3.3 (0.3)	9.1
High-positive pool	6.33	30	6.6 (0.4)	6.0

SD: standard deviation.