RNA Interference Mediated Interleukin-1β Silencing in Inflamed Chondrocytes Decreases Target and Downstream Catabolic Responses

Figure 1

Changes in expression of IL-1β in chondrocytes 24 hours following exposure to siRNA and stimulation with LPS (50 μg/mL). % change (±SEM) is shown relative to control chondrocytes treated with LPS. (a) Comparison of six different IL-1β targeting sequences, all of which show significant decrease in expression. (b) IL-1β expression in LPS-stimulated chondrocytes following transfection with pSilencer expressing shIL-1β #1 and shIL-1β #2. (c) IL-1β expression in LPS-stimulated chondrocytes following transfection with the rAAV transfer plasmid coexpressing tdTomato. Data were analyzed using ANOVA followed by Tukey’s post hoc pairwise analysis. Graph bars with different Tukey classification letters are significantly different at .

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