RNA Interference Mediated Interleukin-1β Silencing in Inflamed Chondrocytes Decreases Target and Downstream Catabolic Responses

Figure 2

Transduction efficiency of rAAV2-tdT-shIL-1β into chondrocytes cultured in monolayer. Fluorescence was quantified using flow cytometry and visually assessed using fluorescence microscopy. (a) Representative flow cytometric plot of untransduced chondrocytes used to set fluorescent gates to account for autofluorescence of chondrocytes. (b) Transduction efficiency assessed by % of chondrocytes expressing the fluorescent protein tdTomato 24, 48, and 72 hours after transduction. (c) Representative flow cytometric plots of transduced chondrocytes 24, 48, and 72 hours following transduction.

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