RNA Interference Mediated Interleukin-1β Silencing in Inflamed Chondrocytes Decreases Target and Downstream Catabolic Responses

Figure 4

Quantitative polymerase chain reaction (qPCR) data showing % change in gene expression of catabolic cytokines and degradative enzymes in control, untransduced (C), LPS-stimulated (LPS), rAAV2-tdT-shIL-1β transduced (shIL-1β), and rAAV2-tdT-shIL-1β transduced and LPS-stimulated chondrocytes (shIL-1β + LPS). % change (±SEM) is shown relative to LPS chondrocytes. shIL-1β + LPS chondrocytes had significantly decreased IL-1β (a), TNF-α (b), and ADAMTS5 expression (c) compared to LPS chondrocytes. Expression of (d) ADAMTS4, (e) MMP3, and (f) MMP13 showed no significant differences between shIL-1β + LPS and LPS chondrocytes. Data were analyzed using ANOVA followed by Tukey’s post hoc pairwise analysis. Graph bars with different Tukey classification letters are significantly different at .

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