Combination of Interleukin-27 and MicroRNA for Enhancing Expression of Anti-Inflammatory and Proosteogenic Genes

Figure 3

Signaling by IL-27. (a) Example of a PathGen analysis. Output analyses using gene expression and luciferase reporter assay data following IL-27 treatment of osteoblasts. IL-27 is formed by EBI3 and IL27p28 subunits, and it binds to receptor IL27RA (WSX1) with gp130. Image cropped in the center and left edge for summarizing the main signaling connections converging in Smad3, Runx2, Stat1, and Stat3, and other connections seen to BMP2, SPP1, DKK1, and WNT10b. Green, upregulated genes; purple, downregulated genes. (b) Reporter luciferase assay with BRE-Luc (BRE motif binds SMAD1/5/8 and responds to BMP family members). Right plot, reporter luciferase assay with SBE4-Luc (SBE motif binds SMAD2/3/4 and responds to TGFB family members) (). OC, differentiating osteoclasts; OB, differentiating osteoblasts.

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