Combination of Interleukin-27 and MicroRNA for Enhancing Expression of Anti-Inflammatory and Proosteogenic Genes

Figure 6

Macrophage cells. RAW264.7 macrophages cultured in the presence of proinflammatory LPS stimulus and cotransfected with miR control or experimental (miRlet7f, miR-10a, miR-20b, miR-21, miR-29b, miR-17, and miR-210) and luciferase reporter vectors for detecting IL17a, TNF, and TRAF2 expression or SMAD2/3, STAT3, and STAT1 activity. (a) Effect of IL-27 (50 ng/ml) on luciferase expression. (b) Effect of experimental miRNA on luciferase expression. (c) Effect of miRNA+IL27 combination on luciferase expression. Black rectangle frames (□) highlight the data with significant expression changes () relative to negative control (miR ctrl); macrophages, RAW264.7 cells treated with activating agent LPS as per Materials and Methods. Yellow arrowheads, proposed best combinations of miR and IL-27. Color bar, range of fold change in expression, from green (−2.0-fold change or decrease) to red (2.4-fold change or increase).