Combination of Interleukin-27 and MicroRNA for Enhancing Expression of Anti-Inflammatory and Proosteogenic Genes

Figure 7

Fibroblastic cells. NIH 3T3 fibroblastic cells grown in the presence of TNF stimulus and cotransfected with miR control or experimental (miRlet7f, miR-10a, miR-20b, or miR-21) and luciferase reporter vectors for detecting IL17a and TNF expression or STAT3 and STAT1 activity. (a) Effect of IL-27 (50 ng/ml) on luciferase expression. (b) Effect of experimental miRNA on luciferase expression. (c) Effect of miRNA+IL27 combination on luciferase expression. Black rectangle frames (□) highlight the data with significant expression changes () relative to negative control (miR ctrl); fibroblastic cells, NIH3T3 cells treated with activating agent TNF as per Materials and Methods. Yellow arrowheads, proposed best combinations of miR and IL-27. Color bar, range of fold change in expression, from green (−2.2-fold change or decrease) to red (2.2-fold change or increase).