Table of Contents
Advances in Toxicology
Volume 2014, Article ID 185201, 11 pages
http://dx.doi.org/10.1155/2014/185201
Research Article

Investigations of the Biological Effects of Airborne and Inhalable Substances by Cell-Based In Vitro Methods: Fundamental Improvements to the ALI Concept

Fraunhofer Institute for Toxicology and Experimental Medicine, Nikolai-Fuchs-Straße 1, 30625 Hannover, Germany

Received 24 July 2014; Accepted 11 October 2014; Published 12 November 2014

Academic Editor: Defeng Wu

Copyright © 2014 Detlef Ritter and Jan Knebel. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. OECD, OECD Guidelines for the Testing of Chemicals, Section 4: Health Effects, 2014. View at Publisher · View at Google Scholar
  2. “ICH Topic S2 (R1) guidance on genotoxicity testing and data interpretation for pharmaceuticals intended for human use,” Tech. Rep. EMA/CHMP/ICH/126642/2008, European Medicines Agency, 2008.
  3. R. F. Phalen, Inhalation Studies, Foundations and Studies, Informa Healthcare, New York, NY, USA, 2nd edition, 2008.
  4. C. Voisin, C. Aerts, E. Jakubczak, J. L. Houdret, and T. B. Tonnel, “Effects of nitrogen dioxide on alveolar macrophages surviving in the gas phase,” Clinical Respiratory Physiology, vol. 13, no. 1, pp. 137–144, 1977. View at Google Scholar · View at Scopus
  5. C. Voisin, C. Aerts, E. Jakubczk, and A. B. Tonnel, “La culture cellulaire en phase gazeuse. Un nouveau modèle expérimental d'étude in vitro des activités des macrophages alvéolaires,” Bulletin Europeen de Physiopathologie Respiratoire, vol. 13, no. 1, pp. 69–82, 1977. View at Google Scholar · View at Scopus
  6. C. Voisin, C. Aerts, J. L. Houdret, A. B. Tonnel, and P. Ramon, “Action of nitrogen dioxide on alveolar macrophages surviving in-vitro in gaseous phase,” Lille Medical, vol. 21, no. 2, pp. 126–130, 1976. View at Google Scholar · View at Scopus
  7. C. Voisin, C. Aerts, A. B. Tonnel, J. L. Houdret, and P. Ramon, “Survival in gaseous phase and reconstitution ‘in vitro’ of the natural micro environment of alveolar macrophages,” Pathologie Biologie, vol. 23, no. 6, pp. 453–459, 1975. View at Google Scholar · View at Scopus
  8. F. Pariselli, M. G. Sacco, J. Ponti, and D. Rembges, “Effects of toluene and benzene air mixtures on human lung cells (A549),” Experimental and Toxicologic Pathology, vol. 61, no. 4, pp. 381–386, 2009. View at Publisher · View at Google Scholar · View at Scopus
  9. R. N. Grass, L. K. Limbach, E. K. Athanassiou, and W. J. Stark, “Exposure of aerosols and nanoparticle dispersions to in vitro cell cultures: a review on the dose relevance of size, mass, surface and concentration,” Journal of Aerosol Science, vol. 41, no. 12, pp. 1123–1142, 2010. View at Publisher · View at Google Scholar · View at Scopus
  10. H.-R. Paur, F. R. Cassee, J. Teeguarden et al., “In-vitro cell exposure studies for the assessment of nanoparticle toxicity in the lung—a dialog between aerosol science and biology,” Journal of Aerosol Science, vol. 42, no. 10, pp. 668–692, 2011. View at Publisher · View at Google Scholar · View at Scopus
  11. L. Müller, P. Comte, J. Czerwinski et al., “New exposure system to evaluate the toxicity of (scooter) exhaust emissions in lung cells in vitro,” Environmental Science and Technology, vol. 44, no. 7, pp. 2632–2638, 2010. View at Publisher · View at Google Scholar · View at Scopus
  12. D. J. Cooney and A. J. Hickey, “Cellular response to the deposition of diesel exhaust particle aerosols onto human lung cells grown at the air-liquid interface by inertial impaction,” Toxicology in Vitro, vol. 25, no. 8, pp. 1953–1965, 2011. View at Publisher · View at Google Scholar · View at Scopus
  13. D. Thorne and J. Adamson, “A review of in vitro cigarette smoke exposure systems,” Experimental and Toxicologic Pathology, vol. 65, no. 7-8, pp. 1183–1193, 2013. View at Publisher · View at Google Scholar · View at Scopus
  14. S. Hein, M. Bur, T. Kolb, B. Muellinger, U. F. Schaefer, and C.-M. Lehr, “The Pharmaceutical Aerosol Deposition Device on Cell Cultures (PADDOCC) in vitro system: design and experimental protocol,” ATLA Alternatives to Laboratory Animals, vol. 38, no. 4, pp. 285–295, 2010. View at Google Scholar · View at Scopus
  15. G. Linsel, M. Bauer, E. Berger-Preiß et al., Prävalidierungsstudie zur Prüfung der Toxischen Wirkung von inhalativ Wirksamen Stoffen (Gase), BAuA Schriftenreihe, Berlin, Germany, 2011.
  16. K. Bérubé, M. Aufderheide, D. Breheny et al., “In Vitro models of inhalation toxicity and disease: the report of a FRAME workshop,” ATLA Alternatives to Laboratory Animals, vol. 37, no. 1, pp. 89–141, 2009. View at Google Scholar · View at Scopus
  17. B. M. Rothen-Rutishauser, S. C. Kiama, and P. Gehr, “A three-dimensional cellular model of the human respiratory tract to study the interaction with particles,” American Journal of Respiratory Cell and Molecular Biology, vol. 32, no. 4, pp. 281–289, 2005. View at Publisher · View at Google Scholar · View at Scopus
  18. L. Lauenstein, S. Switalla, F. Prenzler et al., “Assessment of immunotoxicity induced by chemicals in human precision-cut lung slices (PCLS),” Toxicology in Vitro, vol. 28, no. 4, pp. 588–599, 2014. View at Publisher · View at Google Scholar · View at Scopus
  19. J. E. Nichols, J. A. Niles, S. P. Vega, and J. Cortiella, “Novel in vitro respiratory models to study lung development, physiology, pathology and toxicology,” Stem Cell Research and Therapy, vol. 4, supplement 1, article S7, 2013. View at Publisher · View at Google Scholar · View at Scopus
  20. R. E. Rasmussen, “In vitro systems for exposure of lung cells to NO2 and O3,” Journal of Toxicology and Environmental Health, vol. 13, no. 2-3, pp. 397–411, 1984. View at Google Scholar · View at Scopus
  21. S. Bakand and A. Hayes, “Troubleshooting methods for toxicity testing of airborne chemicals in vitro,” Journal of Pharmacological and Toxicological Methods, vol. 61, no. 2, pp. 76–85, 2010. View at Publisher · View at Google Scholar · View at Scopus
  22. S. Troeller, G. Linsel, N. Huettig et al., “Air/liquid interface (ALI) technique for toxicity testing of gaseous compounds on human cells,” Toxicology Letters, vol. 221, pp. S138–S139, 2013. View at Google Scholar
  23. J. Rach, J. Budde, N. Möhle, and M. Aufderheide, “Direct exposure at the air-liquid interface: evaluation of an in vitro approach for simulating inhalation of airborne substances,” Journal of Applied Toxicology, vol. 34, no. 5, pp. 506–515, 2014. View at Publisher · View at Google Scholar · View at Scopus
  24. S. Weber, M. Hebestreit, T. Wilms, L. L. Conroy, and G. Rodrigo, “Comet assay and air-liquid interface exposure system: a new combination to evaluate genotoxic effects of cigarette whole smoke in human lung cell lines,” Toxicology in Vitro, vol. 27, no. 6, pp. 1987–1991, 2013. View at Publisher · View at Google Scholar · View at Scopus
  25. N. Jeannet, M. Fierz, M. Kalberer, H. Burtscher, and M. Geiser, “Nano aerosol chamber for in-vitro toxicity (NACIVT) studies,” Nanotoxicology, 2014. View at Publisher · View at Google Scholar
  26. D. Thorne, J. Wilson, T. S. Kumaravel, E. D. Massey, and M. McEwan, “Measurement of oxidative DNA damage induced by mainstream cigarette smoke in cultured NCI-H292 human pulmonary carcinoma cells,” Mutation Research—Genetic Toxicology and Environmental Mutagenesis, vol. 673, no. 1, pp. 3–8, 2009. View at Publisher · View at Google Scholar · View at Scopus
  27. B. Rothen-Rutishauser, R. N. Grass, F. Blank et al., “Direct combination of nanoparticle fabrication and exposure to lung cell cultures in a closed setup as a method to simulate accidental nanoparticle exposure of humans,” Environmental Science and Technology, vol. 43, no. 7, pp. 2634–2640, 2009. View at Publisher · View at Google Scholar · View at Scopus
  28. L. C. Chen and Q. Qu, “Formation of intracellular free radicals in guinea pig airway epithelium during in vitro exposure to ozone,” Toxicology and Applied Pharmacology, vol. 143, no. 1, pp. 96–101, 1997. View at Publisher · View at Google Scholar · View at Scopus
  29. M. Savi, M. Kalberer, D. Lang et al., “A novel exposure system for the efficient and controlled deposition of aerosol particles onto cell cultures,” Environmental Science and Technology, vol. 42, no. 15, pp. 5667–5674, 2008. View at Publisher · View at Google Scholar · View at Scopus
  30. J. Rach, B. Halter, and M. Aufderheide, “Importance of material evaluation prior to the construction of devices for in vitro techniques,” Experimental and Toxicologic Pathology, vol. 65, no. 7-8, pp. 973–978, 2013. View at Publisher · View at Google Scholar · View at Scopus
  31. M. Aufderheide, B. Halter, N. Möhle, and D. Hochrainer, “The CULTEX RFS: a comprehensive technical approach for the in vitro exposure of airway epithelial cells to the particulate matter at the air-liquid interface,” BioMed Research International, vol. 2013, Article ID 734137, 15 pages, 2013. View at Publisher · View at Google Scholar · View at Scopus
  32. G. Hendriks, B. Van de Water, W. Schoonen, and H. Vrieling, “Cellular-signaling pathways unveil the carcinogenic potential of chemicals,” Journal of Applied Toxicology, vol. 33, no. 6, pp. 399–409, 2013. View at Publisher · View at Google Scholar · View at Scopus
  33. R. Damoiseaux, S. George, M. Li et al., “No time to lose—high throughput screening to assess nanomaterial safety,” Nanoscale, vol. 3, no. 4, pp. 1345–1360, 2011. View at Publisher · View at Google Scholar · View at Scopus
  34. T. Krucker and B. S. Sandanaraj, “Optical imaging for the new grammar of drug discovery,” Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, vol. 369, no. 1955, pp. 4651–4665, 2011. View at Publisher · View at Google Scholar · View at Scopus
  35. C. Rovida and T. Hartung, “Re-evaluation of animal numbers and costs for in vivo tests to accomplish REACH legislation requirements for chemicals—a report by the transatlantic think tank for toxicology (t4),” Altex, vol. 26, no. 3, pp. 187–208, 2009. View at Google Scholar · View at Scopus
  36. July 2014, http://www.echa.europa.eu/web/guest/chemicals-in-our-life/hot-scientific-topics.