Table of Contents
Advances in Toxicology
Volume 2014 (2014), Article ID 201762, 9 pages
http://dx.doi.org/10.1155/2014/201762
Research Article

Modulation of Tinospora rumphii and Zinc Salt on DNA Damage in Quinoline-Induced Genotoxicity and Hepatotoxicity in Male Albino Mice

1Field of Drug Discovery Research, Faculty of Advanced Life Science, Hokkaido University, N21, W10, Kita-ku, Sapporo 001-0021, Japan
2Chemistry Department, Xavier University-Ateneo de Cagayan, 9000 Cagayan de Oro City, Philippines
3Chemistry Department, College of Science and Mathematics, Mindanao State University-Iligan Institute of Technology, 9200 Iligan City, Philippines

Received 12 August 2014; Accepted 30 October 2014; Published 20 November 2014

Academic Editor: Mugimane Manjanatha

Copyright © 2014 Roger Salvacion Tan and Lydia M. Bajo. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Tinospora rumphii (T. rumphii) is a folkloric medicinal plant that is widely distributed in Asia and Africa. It has been widely used by locals to treat many diseases including jaundice, which is a manifestation of liver damage. We investigated the action of T. rumphii crude extract together with zinc sulphate, a known tumor modulator, on hepatic injuries induced by intraperitoneal (i.p) injections of quinoline on albino mice. The hepatotoxic effect was assessed by bilirubin concentration in the blood serum, while the genotoxic effect was determined by single-cell gel electrophoresis (SCGE). The mice orally fed with the crude extracts, following quinoline exposure, had reduced serum bilirubin concentration and DNA damage. Mice treated with Zinc sulphate, on the other hand, had remarkably reduced DNA damage on hepatocytes. Our findings showed that hepatoprotective potential of T. rumphii extract is dose-dependent and that utilization of the extract as medicinal remedy must be strictly monitored, while zinc was proven to reverse genotoxic effect of quinoline. This study unraveled the potential of T. rumphii extract and zinc as important hepatoprotective agents for future treatment of hepatic damage caused by chemotherapeutic agents used in cancer treatment.