Compound-Specific Toxicities Detected in CFU-GM, Rat Kidney NRK Cells, Rat Bladder RBLAK Cells, and Rat Liver Slices following Batracylin or N-Acetyl Batracylin Exposure

Table 4

% nuclei area positive for γ-H2AX staining.

Treatment	μM	NRK			RBLAK
		6 hr	24 hr	72 hr	6 hr	24 hr	72 hr
Vehicle	0	3.3 ± 0.6	3.0 ± 0.7	1.3 ± 0.1	1.9 ± 0.2	2.0 ± 0.3	2.9 ± 0.6
BAT	3.13	4.8 ± 1.0	5.2 ± 0.2	2.6 ± 0.5	9.5 ± 1.5	12.2 ± 0.3	7.1 ± 1.9
	6.25	5.5 ± 0.7	6.2 ± 0.7	3.4 ± 0.6	8.0 ± 1.4	10.3 ± 0.5	6.3 ± 1.5
	12.5	7.9 ± 0.8	8.9 ± 1.0	5.1 ± 0.5	9.1 ± 1.3	8.8 ± 0.8	6.3 ± 1.6
	25	19.0 ± 2.6	15.9 ± 1.7	6.7 ± 0.8	10.9 ± 1.8	5.2 ± 0.4	7.3 ± 0.7
	50	30.9 ± 3.3	26.7 ± 2.2	8.9 ± 2.1	15.3 ± 1.9	12.1 ± 0.2	1.5 ± 0.4
NAB	0.31	3.6 ± 0.3	2.6 ± 0.5	1.6 ± 0.2	1.5 ± 0.3	1.9 ± 0.1	3.4 ± 0.8
	0.63	3.4 ± 0.6	2.9 ± 0.3	1.4 ± 0.2	1.8 ± 0.5	2.3 ± 0.2	4.5 ± 2.1
	1.25	3.7 ± 0.7	3.2 ± 1.4	1.8 ± 0.4	2.8 ± 0.6	2.1 ± 0.6	4.3 ± 0.9
	2.5	3.7 ± 0.2	4.4 ± 2.3	2.1 ± 0.3	3.2 ± 0.2	2.9 ± 0.6	4.7 ± 0.9
	5	4.3 ± 0.3	3.4 ± 1.0	2.5 ± 0.4	4.8 ± 1.2	4.3 ± 0.7	7.2 ± 2.3
Etoposide	3.13	40.7 ± 1.2	17.9 ± 1.4	13.2 ± 1.5	40.7 ± 2.2	40.9 ± 0.6	34.8 ± 9.0
	25	74.6 ± 4.6	42.0 ± 3.1	27.3 ± 2.8	68.1 ± 2.4	35.5 ± 10.2	19.8 ± 0.9