Bisphenol A Induces Apoptosis in Liver Cells through Induction of ROS

Figure 4

Slide was counterstained with hematoxylin and viewed under a light microscope. Detection of apoptosis TUNEL assay in the liver tissue of rats. The presence of apoptosis after treatment with 2, 10, and 50 mg/kg/bwt of BPA by intraperitoneal injection for 30 days is shown in panel (a). Brown color reaction indicates cells that underwent apoptosis, as the arrows indicate. Quantitative results of the experiment shown in (a), (b), (c), and (d) are depicted in (e) (the graph representing average number of TUNEL-positive cells in each group). Cells were photographed at the 400x magnification. Apoptotic index of liver (%). The symbol represents statistical significant (ANOVA) from control: , , and . Values are represented as the mean ± SD, and for all groups.

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