Human Sperm Bioassay for Reprotoxicity Testing in Embryo Culture Media: Some Practical Considerations in Reducing the Assay Time

Table 1

AAB-compiled PT data on HSA from the reporting of the participating laboratories for the years 2008 and 2009. The value represents motility (mean ± SD) in respective culture conditions at specified observation points.

PT Event	No. of Labs particip	Method type	Obs points	Culture conditions tested
				EC1	EC2	EC1	EC2
				Protein (+)	Protein (+)	Protein (−)	Protein (−)
08−𝐸1	34	Oil overlay	0 Hr	89.6±7.6	89.0±8.2	89.4±7.6	89.5±7.5
08−𝐸1	96	No oil	0 Hr	88.4±9.6	88.1±9.7	86.5±10.7	86.1±11.3
08−𝐸1	142	Combined	0 Hr	88.6±9.5	88.3±9.7	87.1±10.5	87.0±10.6
08−𝐸2	32	Oil overlay	0 Hr	89.7±8.4	89.8±8.2	89.0±10.4	90.4±8.1
08−𝐸2	95	No oil	0 Hr	88.2±9.0	88.1±8.8	86.6±10.3	86.2±11.3
08−𝐸2	138	Combined	0 Hr	88.5±9.2	88.4±9.1	87.1±10.6	87.2±10.9
09−𝐸1	35	Oil overlay	0 Hr	88.6±10.0	88.6±10.1	89.0±9.0	88.7±9.9
09−𝐸1	97	No oil	0 Hr	88.3±8.5	88.4±8.5	87.4±8.6	87.3±8.9
09−𝐸1	140	Combined	0 Hr	88.4±8.7	88.5±8.7	87.8±8.8	87.3±9.5
09−𝐸2	33	Oil overlay	0 Hr	88.3±12.0	89.5±11.0	88.1±13.0	88.2±12.0
09−𝐸2	101	No oil	0 Hr	89.6±7.0	89.8±7.0	88.0±9.0	88.5±9.0
09−𝐸2	143	Combined	0 Hr	89.2±9.0	88.9±9.0	88.2±9.0	88.4±9.0
08−𝐸1	34	Oil overlay	24 Hrs	50.8±25.9	76.4±11.9	45.7±29.1	76.2±12.6
08−𝐸1	94	No oil	24 Hrs	57.2±25.4	78.6±15.1	49.4±25.2	73.8±15.0
08−𝐸1	140	Combined	24 Hrs	55.7±25.9	78.5±14.2	48.0±26.1	73.6±15.3
08−𝐸2	32	Oil overlay	24 Hrs	50.8±29.6	77.3±15.8	43.3±25.9	69.8±19.7
08−𝐸2	92	No oil	24 Hrs	59.2±21.0	79.2±13.0	51.2±23.9	71.9±19.7
08−𝐸2	135	Combined	24 Hrs	56.1±24.1	78.4±13.7	48.3±25.1	71.9±18.5
09−𝐸1	34	Oil overlay	24 Hrs	34.1±26.1	75.5±10.3	29.1±25.4	71.2±12.2
09−𝐸1	96	No oil	24 Hrs	48.5±25.5	81.6±10.3	39.9±25.6	76.9±12.8
09−𝐸1	138	Combined	24 Hrs	44.5±26.4	79.4±11.5	36.8±25.9	75.1±13.2
09−𝐸2	32	Oil overlay	24 Hrs	71.8±15.0	27.7±10.0	69.5±15.0	21.9±10.0
09−𝐸2	100	No oil	24 Hrs	79.7±13.0	50.4±15.0	75.8±13.0	40.6±14.0
09−𝐸2	141	Combined	24 Hrs	79.0±12.0	45.3±10.0	73.6±11.0	38.2±8.0
08−𝐸1	32	Oil overlay	48 Hrs	20.5±23.1	53.0±24.3	14.6±18.0	53.1±26.9
08−𝐸1	91	No oil	48 Hrs	32.1±24.4	62.0±24.0	25.6±22.6	57.5±23.0
08−𝐸1	134	Combined	48 Hrs	29.3±25.1	60.3±24.2	22.8±22.7	55.9±23.7
08−𝐸2	30	Oil overlay	48 Hrs	21.3±21.8	49.1±26.5	15.0±13.7	44.9±24.1
08−𝐸2	90	No oil	48 Hrs	36.9±24.1	66.3±19.0	27.7±22.8	58.5±23.3
08−𝐸2	130	Combined	48 Hrs	32.0±24.4	60.4±23.1	24.4±22.0	55.1±23.6
09−𝐸1	32	Oil overlay	48 Hrs	9.7±11.0	51.2±24.0	8.6±13.1	47.6±26.1
09−𝐸1	91	No oil	48 Hrs	25.4±22.4	62.2±23.8	16.6±17.8	58.3±25.9
09−𝐸1	131	Combined	48 Hrs	22.6±22.3	58.2±24.5	14.8±17.6	54.3±26.4
09−𝐸2	30	Oil overlay	48 Hrs	49.1+14.0	6.8+2.0	43.5+11.0	5.0+1.0
09−𝐸2	95	No oil	48 Hrs	68.4+14.0	22.7+7.0	63.3+14.0	17.7+6.0
09−𝐸2	134	Combined	48 Hrs	61.9+10.0	20.6+4.0	56.1+10.0	14.9+4.0