New Aspects in the Differential Diagnosis and Therapy of Bladder Pain Syndrome/Interstitial Cystitis

Table 2

Primers used for real-time PCR.

Primer	Sequence 5′ → 3′	Product length (bp)	Binding site	AccNo
h36B4 forward	AACATGCTCAACATCTCCCC	397	exon 6	NR_002775.1
h36B4 reverse	CCGACTCCTCCGACTCTTC		exon 8
aSMCA forward	CCAACTGGGACGACATGGAAA	212	exon 4	NM_001613.2
aSMCA reverse	GCGTCCAGAGGCATAGAGAGACA		exon 6
M2 forward	CTAAGCAAACATGCATCAGAATTGG	288	exon 6	NM_001006632.1
M2 reverse	AAGGTGCACAAAAGGTGTTAATGAG		exon 6
M3 forward	ACCCAGCTCCGAGCAGATGGAC	341	exon 5	NM_000740.2
M3 reverse	CGGCTGACTCTAGCTGGATGGG		exon 5
P2×1 forward	GCGTAATAAGAAGGTGGGCGTTA	109	exon 1	NM_002558.2
P2×1 reverse	GCCGCTCGAGGTCTGGTA		exon 2
P2×2 forward	CAGGTTTGCCAAATACTACAAGATCA	105	exon 8	NM_174873.1
P2×2 reverse	AACTTCCCGGCCTGTCCAT		exon 9
P2×3 forward	TCTTCACCTATGAGACCACCAAGTC	83	exon 1	NM_002559
P2×3 reverse	GATCAGAAGCTGAACTACTCGGTTGAT		exon 1
H1 forward	AAGTCACCATCCCAAACCCCCAAG	151	exon 3	NM_001098213
H1 reverse	TCAGGCCCTGCTCATCTGTCTTGA		exon 3
H2 forward	AGGAACGAGACCAGCAAGGGCAAT	198	exon 2a	NM_022304
H2 reverse	GGTGGCTGCCTTCCAGGAGCTAAT		exon 2a

h36B4 = human acidic ribosomal protein P0; aSMCA = alpha-smooth muscle cell actin.