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Advances in Urology
Volume 2016, Article ID 1282531, 8 pages
http://dx.doi.org/10.1155/2016/1282531
Research Article

Novel Bioceramic Urethral Bulking Agents Elicit Improved Host Tissue Responses in a Rat Model

1Department of Surgery, University of Vermont, Burlington, VT 05405, USA
2Department of Mechanical Engineering and Engineering Science, University of North Carolina at Charlotte, Charlotte, NC 28223, USA
3Department of Medicine, Philipps University of Marburg, 35039 Marburg, Germany
4Department of Pathology and Laboratory Medicine, University of Vermont, Burlington, VT 05405, USA
5TAK Biopharma, 2000 Frederiksberg, Denmark
6Department of Surgical Sciences, Ostrava University, 703 00 Ostrava, Czech Republic
7Department of Urology and Biomedical Laboratory, Clinical Institute, University of Southern Denmark, 5000 Odense, Denmark

Received 1 June 2016; Accepted 31 July 2016

Academic Editor: Matthew Rutman

Copyright © 2016 Travis K. Mann-Gow et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objectives. To test the physical properties and host response to the bioceramic particles, silica-calcium phosphate (SCPC10) and Cristobalite, in a rat animal model and compare their biocompatibility to the current clinically utilized urethral bulking materials. Material and Methods. The novel bulking materials, SCPC10 and Cristobalite, were suspended in hyaluronic acid sodium salt and injected into the mid urethra of a rat. Additional animals were injected with bulking materials currently in clinical use. Physiological response was assessed using voiding trials, and host tissue response was evaluated using hard tissue histology and immunohistochemical analysis. Distant organs were evaluated for the presence of particles or their components. Results. Histological analysis of the urethral tissue five months after injection showed that both SCPC10 and Cristobalite induced a more robust fibroblastic and histiocytic reaction, promoting integration and encapsulation of the particle aggregates, leading to a larger bulking effect. Concentrations of Ca, Na, Si, and P ions in the experimental groups were comparable to control animals. Conclusions. This side-by-side examination of urethral bulking agents using a rat animal model and hard tissue histology techniques compared two newly developed bioactive ceramic particles to three of the currently used bulking agents. The local host tissue response and bulking effects of bioceramic particles were superior while also possessing a comparable safety profile.