Histoprotective Effect of Essential Oil from Citrus aurantifolia in Testosterone-Induced Benign Prostatic Hyperplasia Rat

Figure 8

Photomicrographs of H&E stained liver sections of experimental groups: (a) sham; (b) model, TE (10 mg/kg)/olive oil injection (i.m.); (c) finasteride, positive control, TE (10 mg/kg)/olive oil injection (i.m.) + finasteride administration (15 mg/kg, p.o.); (d) small dose, TE (10 mg/kg)/olive oil injection (i.m.) + LEO (30 mg/kg); (e) medium dose, TE (10 mg/kg)/olive oil injection (i.m.) + LEO (100 mg/kg); (f) high dose, TE (10 mg/kg)/olive oil injection (i.m.) + LEO (300 mg/kg). Black arrow represents sinusoids separating plates of hepatocytes; red arrow represents Kupffer cells; Yellow arrow represents hepatocytes with fat accumulation, the orange arrow represents inflammatory cells, and CV represents centrilobular vein. The model group showed enlarged hepatocytes with eosinophilic or pale staining cytoplasm or enlarged nucleus and enlarged sinusoids infiltrated with Kupffer cells and inflammatory cells. Finasteride-treated group had dilated sinusoids with few inflammatory cells and normal sized hepatocytes with slightly enlarged nuclei with a prominent nucleolus. Lime essential oil-treated groups showed dose-dependent decrease in sinusoids infiltrated with Kupffer cells or inflammatory cells, dose-dependent increase in hepatocyte fat accumulation. High dose treated group had a presentation close to the sham group.

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