Novel Roles of the Picornaviral 3D Polymerase in Viral Pathogenesis
Figure 3
Viral transcripts and demyelination in mice infected with TMEV. (a) Nontransgenic and 3D transgenic mice were infected with plaque forming units of TMEV intracerebrally. Brain and spinal cord were harvested after 7 and 180 days and total RNA was isolated using Trizol reagent (Invitrogen). Viral transcripts for TMEV were quantified by real-time RT-PCR using primers specific for the VP2 region of TMEV. Data are expressed as mean relative viral transcripts + SEM ( for nontransgenic and 5 for 3D transgenic) in the brain or spinal cord above uninfected mice and were normalized to GAPDH. (b) Percent of spinal cord quadrants containing demyelinated lesions in 3D transgenic and nontransgenic FVB mice at 270 days after infection. Statistical significance was determined using the Mann-Whitney rank sum test.
