In-Depth Global Analysis of Transcript Abundance Levels in Porcine Alveolar Macrophages Following Infection with Porcine Reproductive and Respiratory Syndrome Virus
Table 2
(a) Changes in chemokine transcript abundance in PRRSV-infected PAMs
Transcript
Function
RT-PCR
Normalized SAGE tag counts (per million tags)
Mock
6
12
16
24
CCL3 (MIP1α)
Chemotaxis
191
21
3
0
21
CCL4 (MIP1β)
Chemotaxis
419
68
25
19
16
CCL5 (RANTES)
Chemotaxis
nd
7
7
17
23
13
MIF
Inhibits macrophage migration
nd
8
1
25
35
32
CCL20 (MIP3α)
Chemotaxis
nd
196
78
14
19
11
CCL2 (MCP-1)
Chemotaxis
nd
1
1
1
4
31
CCL7 (MCP-3)
Chemotaxis
nd
0
0
4
1
22
CXCL2 (GROβ)
Chemotaxis
1596
659
335
215
155
CXCL8 (AMCF-1/IL-8)*
Chemotaxis
6 hr
353 8
807 172
370 121
202 55
449 17
CXCL5 (AMCF-2)
Chemotaxis
6 hr
0
88
31
13
80
(b) Changes in cytokine transcript abundance in PRRSV-infected PAMs
Transcript
Function
RT-PCR
Normalized SAGE tag counts (per million tags)
Mock
6
12
16
24
IL-6
Acute phase response, T- and B-cell growth & differentiation
No tag in transcript
GM-CSF
Dendritic cell growth & differentiation
nd
4
3
1
0
4
TNF-α
Local inflammation, endothelial activation
nd
49
3
1
0
0
IL-1α
T-cell activation, macrophage activation
152
31
23
16
40
Il-1β
T-cell activation, macrophage activation
283
103
31
52
163
(c) Changes in Immediate Early-Response Transcript Abundance in PRRSV-infected PAMs
