Artificial 64-Residue HIV-1 Enhancer-Binding Peptide Is a Potent Inhibitor of Viral Replication in HIV-1-Infected Cells

Figure 10

Determination of the 50% effective dose of peptide R64 based on R64 concentration-dependent HIV-1 RNA levels. Using the CalcuSyn 1.1 software package (Biosoft) [39], a median-effect plot was derived from the dose-effect curve obtained at day 8 after infection using the equation: 𝑓_𝑎/𝑓_𝑢=(𝐷/𝐷_𝑚)^𝑚 where 𝐷 is the dose, 𝐷_𝑚 is the dose required for a 50% effect, 𝑓_𝑎 is the fraction affected by the dose, 𝑓_𝑢 is the unaffected fraction, 𝑚 is a coefficient of the sigmoidicity of the dose-effect curve, and 𝑟 is the correlation coefficient (𝐷_𝑚=0.588 μM, 𝑟=0.8249, 𝑚=−1.320).