Artificial 64-Residue HIV-1 Enhancer-Binding Peptide Is a Potent Inhibitor of Viral Replication in HIV-1-Infected Cells

<table>Western blot of cytosolic and nuclear extracts of R64-containing CHO-K1 cells. R64 was detected using rabbit anti-R42 antiserum, alkaline phosphatase-conjugated goat anti-rabbit IgG, and the phosphatase substrate 5-bromo-4-chloro-3-indolyl phosphate. Lane 1: molecular mass markers; lane 2: synthetic R64 (calculated molecular mass, 7611 Da; 500 ng applied); lanes 3 and 7: nuclear and cytosolic cell extract controls; nuclear and cytosolic extracts of cells incubated with 1.3 <i>μ</i>M R64 (lanes 4 and 8), 1.7 <i>μ</i>M R64 (lanes 5 and 9), and 2.3 <i>μ</i>M R64 (lanes 6 and 10), respectively.</table>

Advances in Virology

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Figure 5

Figure 5: Artificial 64-Residue HIV-1 Enhancer-Binding Peptide Is a Potent Inhibitor of Viral Replication in HIV-1-Infected Cells 