- About this Journal ·
- Abstracting and Indexing ·
- Aims and Scope ·
- Article Processing Charges ·
- Articles in Press ·
- Author Guidelines ·
- Bibliographic Information ·
- Citations to this Journal ·
- Contact Information ·
- Editorial Board ·
- Editorial Workflow ·
- Free eTOC Alerts ·
- Publication Ethics ·
- Reviewers Acknowledgment ·
- Submit a Manuscript ·
- Subscription Information ·
- Table of Contents
Advances in Virology
Volume 2011 (2011), Article ID 249640, 9 pages
Replication of Alphaviruses: A Review on the Entry Process of Alphaviruses into Cells
Laboratory of Molecular RNA Virology and Antiviral Strategies, Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System, 5 Science Drive 2, National University of Singapore, Singapore 117597
Received 31 January 2011; Accepted 3 May 2011
Academic Editor: Jay C. Brown
Copyright © 2011 Jason Yat-Sing Leung et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Alphaviruses are small, enveloped viruses, ~70 nm in diameter, containing a single-stranded, positive-sense, RNA genome. Viruses belonging to this genus are predominantly arthropod-borne viruses, known to cause disease in humans. Their potential threat to human health was most recently exemplified by the 2005 Chikungunya virus outbreak in La Reunion, highlighting the necessity to understand events in the life-cycle of these medically important human pathogens. The replication and propagation of viruses is dependent on entry into permissive cells. Viral entry is initiated by attachment of virions to cells, leading to internalization, and uncoating to release genetic material for replication and propagation. Studies on alphaviruses have revealed entry via a receptor-mediated, endocytic pathway. In this paper, the different stages of alphavirus entry are examined, with examples from Semliki Forest virus, Sindbis virus, Chikungunya virus, and Venezuelan equine encephalitis virus described.
Alphaviruses are primarily arthropod-borne viruses (arboviruses) within the family Togaviridae. Viruses belonging to this genus are often classified as either New World alphaviruses, or Old World alphaviruses, depending on the geographic location from which they were originally isolated . New World alphaviruses, including Eastern equine encephalitis virus (EEEV), Venezuelan equine encephalitis virus (VEEV) and Western equine encephalitis virus (WEEV), typically cause encephalitis in humans and other mammals, whereas Old World alphaviruses, such as Chikungunya virus (CHIKV), O’Nyong-Nyong virus (ONNV), Ross River virus (RRV), Semliki Forest virus (SFV), and Sindbis virus (SINV), cause a fever, rash, and arthralgia syndrome that rarely causes fatality . While early studies on alphaviruses focused on the prototypic SFV and SINV due to their ability to grow to high titres in cell culture while being nonpathogenic to humans, recent attention has been directed towards investigating CHIKV. The 2005 outbreak of CHIKV in La Reunion infected 40% of the 785,000 population, resulting in 250 fatal cases . The reemergence of CHIKV reiterates the potential threat that alphaviruses pose to human health, and the necessity to understand mechanisms involved in alphavirus biology.
2. Genomic Composition and Virion Structure
Alphaviruses are small, icosahedral-shaped, enveloped viruses, approximately 70 nm in diameter [4–6]. The alphavirus virion has a host-cell acquired lipid membrane [6–9]. Embedded within this membrane are 80 spikes, arranged in a icosahedral [6, 9]. The glycoproteins E1 and E2 associate as heterodimer subunits, which are in turn assembled into trimers to form the spike protrusions [9–11]. Both E1 and E2 are transmembrane proteins with C-terminal cytoplasmic regions that are thought to interact with the nucleocapsid [12, 13].
The alphavirus genome is a single-stranded, positive-sense, RNA genome approximately 12 Kb in length [14, 15]. In addition to genomic length RNA, subgenomic RNA encoding the structural proteins is also generated, with both species containing a 5′ cap and a poly(A) tail [14–16]. The coding sequence consists of two large open reading frames (ORFs); the N-terminal ORF encodes the nonstructural polyprotein while the C-terminal ORF encodes the structural polyprotein (Figure 1). The two polyproteins are cleaved posttranslationally by viral (cysteine) and host proteases. The four nonstructural proteins (nsP1 to 4) and their cleavage intermediates are involved in RNA replication, with the five structural proteins (C, E3, E2, 6K, E1) and their cleavage intermediates required for viral encapsidation and budding (Figure 1) [15, 17, 18].
The alphavirus nsP1 possesses both guanine-7-methyltransferase and guanylyl transferase activities required for capping and methylation of newly synthesized viral genomic and subgenomic RNAs [19, 20]. During RNA replication, nsP1 is thought to anchor replication complexes to cellular membranes . The alphavirus nsP2 exhibits RNA triphosphatase/nucleoside triphosphatase, as well as helicase activity within the N-terminal half [22–24] while the C-terminal half encodes the viral (papain-like) cysteine protease required for processing of the nonstructural polyprotein [17, 25]. Crystal structures of the CHIKV and VEEV nsP3 N-terminus indicate ADP-ribose 1-phosphate phosphatase and RNA-binding activity  while mutagenesis studies also reveal a role for nsP3 in modulating pathogenicity in mice [27, 28]. The nsP4 protein functions as the RNA-dependent RNA-polymerase (RdRp), containing the catalytic GDD motif in the C-terminus . It has also been hypothesized that nsP4 acts as a scaffold for interaction with other nsPs or host proteins via its N-terminal , with adenylyl transferase activity also observed .
During nucleocapsid formation, the alphavirus capsid protein (C) binds viral genomic RNA via N-terminal Arg, Lys, and Pro residues [32, 33]. Mutagenesis studies identified a leucine zipper located within this region essential for formation of nucleocapsid-like particles, presumably mediating dimerization during virus assembly . The protein C-terminal is the serine-protease domain [18, 35], which also contains a hydrophobic pocket for glycoprotein binding adjacent to the substrate-binding site . The role of the structural protein E3 is currently undefined, and appears to vary between different alphaviruses. While the E3 protein of SFV is found associated with virions , the E3 protein is not incorporated into virions of other alphaviruses including CHIKV, SINV, or WEEV . The E2 glycoprotein of alphaviruses responsible for receptor binding is embedded within the membrane courtesy of 30 C-terminal residues [38–40]. Amino acid changes identified the E2 protein as a determinant of neurovirulence [41–43]. Site-directed mutagenesis identified an Tyr-X-Leu tripeptide within the endodomain required for interaction with the capsid protease domain [12, 13, 44], in concert with conserved Cys residues that are modified by palmitoylation . 6K is a palmitoylated structural protein essential for alphavirus particle assembly [46, 47], where it is thought to influence transport to sites of virion assembly at the plasma membrane, before being incorporated into virions in small amounts [46, 48, 49]. The alphavirus 6K protein has also been classified as a viroporin due to its ability to form cation-selective ion channels and alter membrane permeability in bacterial and mammalian cells [50–52]. The E1 protein is the alphavirus fusion protein [53, 54], with a fusion peptide residing within a highly conserved hydrophobic domain .
3. Alphavirus Life-Cycle
Upon entry, alphavirus particles undergo disassembly, releasing genomic RNA into the cytoplasm of infected cells (Figure 2). The viral genome is then translated from two ORFs to generate the nonstructural (P1234) and structural polyproteins . Early in infection P1234 is cleaved in cis between nsP3 and nsP4 to yield P123 and nsP4 [56, 57]. P123 and nsP4 form an unstable initial replication complex, which is able to synthesize negative-strand RNA [1, 58–60]. Cleavage of P123 to nsP1 and P23 can only occur in trans, and only at a sufficiently high concentration of the polyprotein. The polyprotein products nsP1, P23, and nsP4 form a replication complex within virus-induced cytopathic vacuoles (CPV I) that are active in negative-strand synthesis, as well as genomic RNA synthesis, but not in subgenomic RNA synthesis [60–64]. After complete cleavage to nsP1, nsP2, nsP3, and nsP4, negative-strand synthesis is inactivated and the now stable replication complex switches to synthesis of positive-strand genomic and subgenomic RNA [58, 59]. In most alphaviruses, a leaky termination codon is present following nsP3 (indicated in Figure 2), with read-through estimated to occur with only 10–20% efficiency . This leads to an excess of P123 nonstructural polyprotein compared to P1234, and a depletion of nsP4 relative to the other nsPs. Further diminishing intracellular nsP4 is a destabilizing tyrosine residue at the N-terminal which signals rapid degradation by the N-end rule pathway . It is worth noting that expression of a small fraction of nsP4 in cells is relatively stable, presumably in the form of replication complexes, suggesting nsP4 is only degraded when in excess . Removal of the destabilizing Tyr residue leads to poor RNA replication .
Cleavage of the structural polyprotein occurs cotranslationally, beginning with the autoproteolytic cleavage of the capsid protein from the remainder of the polyprotein [18, 68, 69]. C protein is then available to associate with newly synthesized RNA, recognizing specific packaging signals in the 5′ half of the genome, such that only full-length genomic RNA is packaged into nucleocapsid-like particles [32, 33]. The E3 protein acts as a signal sequence for insertion of the remaining polyprotein into the endoplasmic reticulum, where it is processed by host signal peptidase [18, 70]. Similarly, the 6K protein acts as a signal sequence for the downstream processing of the E1 protein .
Upon synthesis, the E2 glycoprotein precursor, PE2 (p62 in SFV), and E1 glycoproteins interact with each other (preferentially in cis) to form heterodimers [71–73]. These heterodimer complexes are then transported from the endoplasmic reticulum to the cell surface via the Golgi complex [74–76]. At a late stage of transport, the PE2 precursor is cleaved in its lumenal domain by host furin-like protease to generate mature E2 and E3 proteins [74, 76, 77]. This cleavage induces a conformational change that weakens the E1-E2 interaction in the spike heterodimer , priming the fusion peptide for activation upon exposure to low pH . Interactions between the C protein and the cytoplasmic domain of the E2 protein drive the budding process, with E1-E2 heterodimers forming an envelope around nucleocapsid-like particles [12, 79, 80]. Upon release from cells, virions acquire a membrane bilayer derived from the host cell plasma membrane [6–9].
4. Receptor-Mediated Endocytosis
Viruses enter cells at the plasma membrane, either by fusion with membrane components at the cell surface, or by receptor attachment and internalization, followed by fusion with intracellular membranes of endocytic vesicles. Receptor-mediated endocytosis is the predominant mode of entry, most often mediated by the formation of clathrin-coated pits, and the subsequent transport to early endosomes, where the low-pH environment triggers fusion . Alternatively, some viruses utilise clathrin-independent pathways to gain entry into cells. The caveolar/raft pathway transports internalised virus to neutral-pH caveosomes, before redistribution to the ER. There are also a number of clathrin-independent, caveolin-independent pathways that viruses use for cellular entry that rely on small GTPases, although these are not well understood .
The entry of alphaviruses into cells is facilitated by interaction of the spike E2 component with protein receptors on the surface of target cells (Figure 2) [40, 82]. A 63 KDa protein on the surface of avian cells was the first alphavirus receptor observed, although its identity was not determined . Antibodies generated against BHK membrane proteins were screened to identify the 67 KDa laminin receptor as a high-affinity attachment receptor for SINV infection in mammalian cells . Subsequent binding experiments of SINV to dendritic cells was shown to be SIGN dependent, with DC-SIGN and L-SIGN acting as receptor molecules . Heparan sulfate, a cell surface glycosaminoglycan, may also act as an attachment receptor for alphaviruses [86–88]. However, the affinity of alphavirus binding to heparan sulfate seems to be acquired after serial passaging in cell culture, with field isolates displaying much lower affinity for heparan sulfate than laboratory-adapted strains [86–88].
Upon attachment to cellular receptors, alphaviruses are rapidly internalized and delivered to endosomes (Figure 2) [89–91]. The formation of clathrin-coated vesicles requires dynamin, a ~100 KDa protein that facilitates the budding of clathrin-coated pits, leading to the formation of coated vesicles, in a GTP-dependent manner . When dominant-negative dynamin mutants specifically blocking the formation of clathrin-coated pits and vesicles were expressed [93, 94], entry of the alphaviruses CHIKV, SFV, and SINV were prevented [89, 95]. Similarly, a dominant-negative mutant of Eps15, another mediator of clathrin-dependent endocytosis, prevents entry of VEEV into cells . Investigations performed using dominant-negative mutant forms of Rab5 and Rab7, genes important in endocytic trafficking to the early and late endosomes, respectively [97, 98], indicate that both SFV and VEEV are transported to early endosomes whereas only VEEV is transported to late endosomes, before fusion with target membranes [96, 99].
While the entry of alphaviruses is widely accepted to be dependent on clathrin-mediated endocytosis and fusion with endosomal membranes, the ability of alphaviruses to enter host cells via alternative mechanisms has also been reported. Supporting this hypothesis is an early study on SINV entry that showed the translation of viral RNA in the cytosol of cells, even when infected cells were treated with the weak bases chloroquine and ammonium chloride, suggesting infection involving acidic endosomes can be circumvented [100, 101]. More recently, the infection of various cell lines by SINV was shown to proceed in the absence of low-pH-induced endocytosis, indicative of entry via a clathrin-independent pathway [102–104]. Similarly, SFV infection of BHK and CHO cells following either normal virus fusion in endosomes, or experimentally induced fusion at the cell surface, highlighted the ability of alphaviruses to infect cells by an alternative pathway. Although CHO cells could only be infected following the endocytic pathway, BHK cells were able to be infected efficiently following fusion in either endosomes or at the plasma membrane, as evidenced by viral RNA and protein synthesis . In agreement with this was the finding that SFV could be found inside noncoated pits and vesicles . When siRNA was used to knock-down clathrin heavy chain, CHIKV infection of both HEK293 and HeLa cells was unaffected . Interestingly, experiments using anti-clathrin antibodies showed only a ~60% block in SFV infection . This partial block in infection could mean that either the antibodies do not inhibit the clathrin pathway completely, or that SFV can also enter through an alternative pathway that does not require clathrin. Such a scenario may also be true for CHIKV, where dominant-negative mutants of Eps15, Rab5, and drug inhibitors of endocytosis, showed only partial block in infection, supporting the hypothesis that several pathways are hijacked by CHIKV to penetrate into target cells .
Early studies revealed the E1 protein of alphaviruses to be the fusion protein [38, 53, 54, 108–110]. Furthermore, removal of the E2 protein by protease digestion suggested that the E1 protein alone is sufficient for membrane fusion to occur . However, fusogenic activity of the E1 protein is suppressed by interaction with the E2 protein . Within endosomal vesicles, the E1-E2 heterodimer undergoes irreversible conformational changes upon exposure to pH of ~6 or below [91, 109, 112–115]. This low-pH environment liberates the E1 subunit from association with the E2 subunit, allowing the rearrangement to a homotrimeric complex active for fusion [108, 109, 116, 117]. E1 homotrimers associate with the target membrane via membrane insertion of the hydrophobic fusion peptide to form pores in both cellular and viral membranes for release of nucleocapsid into the cytoplasm (Figure 2) [111, 118, 119]. The fusion process occurs very rapidly, before alphaviruses are at risk of lysosomal degradation . Treatment of cells with lysosomotropic weak bases chloroquine, concanamycin, ammonium chloride, bafilomycin, or monensin neutralizes the pH in endosomes, preventing fusion with membranes [91, 96, 99, 107, 120, 121].
In addition to a dependence on low pH, the fusion of alphaviruses to membranes requires the presence of cholesterol [107, 108, 114, 115, 122–125]. Small amounts of sphingolipid are also required in target membranes for an as yet unidentified role during the fusion reaction itself [114, 123, 126]. Cholesterol appears to be necessary for the hydrophobic interaction of the alphavirus E1 ectodomain with the target membrane leading up to fusion [113, 127]. However, this cholesterol-dependence differs amongst the alphaviruses; while the entry of CHIKV, SFV, and SINV is inhibited by the depletion of cholesterol, VEEV is still able to enter cells under similar conditions [96, 107]. It was proposed that variations between the envelope proteins of VEEV compared to other alphaviruses may account for these observed differences . As predicted, cholesterol dependence of SFV and SINV were attributed to a specific residue within the E1 protein at position 226 . Viruses containing an E1-P226S mutation are more efficient at fusion in the absence of cholesterol compared to wild-type, with the E1 protein converting to the active fusion homotrimer more readily [128, 129]. Sequence analysis of the VEEV E1 protein shows that this mutation is already present . It has been shown that the membranes of early endosomes are enriched in cholesterol whereas late endosomes are depleted of membrane cholesterol [130, 131]. This has been used to explain the differences in cholesterol requirement, since SFV appears to undergo fusion in early endosomes while VEEV traffics to late endosomes, for fusion .
6. Nucleocapsid Disassembly
Due to steric hindrance, only a small fraction of the E1 fusion protein molecules present on the surface of an individual virus particle would be able to participate in fusion reactions within the endosomal membrane . It has been proposed that the remaining fusion proteins that have not reacted with the target membrane may fold back and react with the viral membrane in which they are anchored, leading to the formation of ion-permeable pores . Together, these processes allow the delivery of nucleocapsid into the cytoplasm (Figure 2), and a flow of ions through the target membrane.
The formation of ion-permeable pores by viral proteins during entry has been found with other viruses such as influenza virus, with the M2 protein implicated in the flow of protons from the endosome at low pH . A similar function for the alphavirus E1 protein has been proposed [111, 119]. Indeed, the accumulation of viral structural proteins in the cell membrane during virus multiplication alters the permeability of the membrane at low pH late in infection [118, 134]. When membrane permeability of cells incubated with SINV and SFV at low pH was assessed, voltage measurements confirmed the formation of ion-permeable pores . The resulting flow of protons from the endosome into the cytoplasm through this pore would lead to a region of low pH, consistent with the discovery that a low-pH environment strongly stimulates disassembly of alphavirus nucleocapsid .
Fusion of the viral envelope with the endosomal membrane releases nucleocapsid into the cell cytoplasm (Figure 2). Uncoating of alphavirus nucleocapsids occurs almost immediately (~1 minute) after their penetration into the cytoplasm . 60S ribosomal RNA interacts with the C protein, facilitating uncoating of the nucleocapsid and release of viral RNA for initiation of protein synthesis (Figure 2) [55, 137–139].
The study of alphaviruses has provided a number of insights relating to virus entry. In fact, reports on SFV were the first to demonstrate entry of viruses via receptor-mediated endocytosis, and the use of clathrin-coated vesicles . The entry pathway of alphaviruses has been further elucidated, providing a greater understanding of events such as virus trafficking, fusion, and the release of genomic material into cells. However, there remain a number of questions that are yet to be resolved. One area for investigation is the identification of receptors used in alphavirus attachment to cells. So far, receptors have only been identified for SFV and SINV entry into cells. Isolating receptors could lead to the development of novel antivirals targeting alphavirus entry. Another topic for exploration is the use of alternative pathways for alphavirus entry into cells. There is growing evidence that alphaviruses are able to infect cells independent of clathrin-mediated endocytosis, either by employing a different entry pathway altogether, or being able to enter cells using several pathways. In the case of CHIKV, entry has also been shown to occur in the absence of cav-1 (required for caveolar vesicle formation) . It may be possible that alphaviruses such as CHIKV are able to utilize a dynamin-dependent pathway reliant on the small GTPase RhoA . This pathway does not involve clathrin, caveolae, or Eps15, yet is strongly inhibited by knock-out of dynamin, RhoA, or the depletion of cholesterol or sphingolipids, concurring with previous work. It is hoped that the continued study of alphaviruses will shed new light on the processes involved in entry.
NUS Start-up Grant (R182-000-165-133, R182-000-165-733) and Biomedical Research Council (BMRC) Research Grant (R182-000-158-305) are acknowledged. Due to space limitations, recognition and apologies are given in advance to the many colleagues whose original contributions have not been possible to cite in this curent review.
- J. H. Strauss and E. G. Strauss, “The alphaviruses: gene expression, replication, and evolution,” Microbiological Reviews, vol. 58, no. 3, pp. 491–562, 1994.
- K. D. Ryman and W. B. Klimstra, “Host responses to alphavirus infection,” Immunological Reviews, vol. 225, no. 1, pp. 27–45, 2008.
- F. Simon, H. Tolou, and P. Jeandel, “The unexpected Chikungunya outbreak,” Revue de Medecine Interne, vol. 27, no. 6, pp. 437–441, 2006.
- E. J. Mancini, M. Clarke, B. Gowen, T. Rutten, and S. D. Fuller, “Cryo-electron microscopy reveals the functional organization of an enveloped virus, Semliki Forest virus,” Molecular Cell, vol. 5, no. 2, pp. 255–266, 2000.
- C. Morgan, C. Howe, and H. M. Rose, “Structure and development of viruses as observed in the electron microscope. V. Western equine encephalomyelitis virus,” The Journal of Experimental Medicine, vol. 113, pp. 219–234, 1961.
- S. D. Fuller, “The T=4 envelope of sindbis virus is organized by interactions with a complementary T=3 capsid,” Cell, vol. 48, no. 6, pp. 923–934, 1987.
- N. H. Acheson and I. Tamm, “Replication of semliki forest virus: an electron microscopic study,” Virology, vol. 32, no. 1, pp. 128–143, 1967.
- R. A. Laine, H. Söderlund, and O. Renkonen, “Chemical composition of Semliki Forest virus,” Intervirology, vol. 1, no. 2, pp. 110–118, 1973.
- R. H. Vogel, S. Provencher, and C. H. von Bonsdorff, “Envelope structure of Semliki Forest virus reconstructed from cryo-electron micrographs,” Nature, vol. 320, no. 6062, pp. 533–535, 1986.
- C. M. Rice and J. Strauss, “Association of Sindbis virion glycoproteins and their precursors,” Journal of Molecular Biology, vol. 154, no. 2, pp. 325–348, 1982.
- J. Wahlberg, W. A. M. Boere, and H. Garoff, “The heterodimeric association between the membrane proteins of Semliki Forest virus changes its sensitivity to low pH during virus maturation,” Journal of Virology, vol. 63, no. 12, pp. 4991–4997, 1989.
- K. E. Owen and R. J. Kuhn, “Alphavirus budding is dependent on the interaction between the nucleocapsid and hydrophobic amino acids on the cytoplasmic domain of the E2 envelope glycoprotein,” Virology, vol. 230, no. 2, pp. 187–196, 1997.
- H. Zhao, B. Lindqvist, H. Garoff, C. H. von Bonsdorff, and P. Liljeström, “A tyrosine-based motif in the cytoplasmic domain of the alphavirus envelope protein is essential for budding,” The EMBO Journal, vol. 13, no. 18, pp. 4204–4211, 1994.
- D. T. Simmons and J. Strauss, “Replication of Sindbis virus. I. Relative size and genetic content of 26 s and 49 s RNA,” Journal of Molecular Biology, vol. 71, no. 3, pp. 599–613, 1972.
- E. G. Strauss, C. M. Rice, and J. Strauss, “Complete nucleotide sequence of the genomic RNA of Sindbis virus,” Virology, vol. 133, no. 1, pp. 92–110, 1984.
- R. Cancedda and A. J. Shatkin, “Ribosome-protected fragments from sindbis 42-S and 26-S RNAs,” European Journal of Biochemistry, vol. 94, no. 1, pp. 41–50, 1979.
- W. R. Hardy and J. Strauss, “Processing the nonstructural polyproteins of Sindbis virus: nonstructural proteinase is in the C-terminal half of nsP2 and functions both in cis and in trans,” Journal of Virology, vol. 63, no. 11, pp. 4653–4664, 1989.
- P. Melançon and H. Garoff, “Processing of the Semliki Forest virus structural polyprotein: role of the capsid protease,” Journal of Virology, vol. 61, no. 5, pp. 1301–1309, 1987.
- T. Ahola and L. Kääriäinen, “Reaction in alphavirus mRNA capping: formation of a covalent complex of nonstructural protein nsP1 with 7-methyl-GMP,” Proceedings of the National Academy of Sciences of the United States of America, vol. 92, no. 2, pp. 507–511, 1995.
- S. Mi and V. Stollar, “Expression of Sindbis virus nsP1 and methyltransferase activity in Escherichia coli,” Virology, vol. 184, no. 1, pp. 423–427, 1991.
- J. Peränen, P. Laakkonen, M. Hyvönen, and L. Kääriäinen, “The alphavirus replicase protein nsP1 is membrane-associated and has affinity to endocytic organelles,” Virology, vol. 208, no. 2, pp. 610–620, 1995.
- M. Gómez de Cedrón, N. Ehsani, M. L. Mikkola, L. Kääriäinen, and J. A. García, “RNA helicase activity of Semliki Forest virus replicase protein NSP2,” The FEBS Letters, vol. 448, no. 1, pp. 19–22, 1999.
- M. Rikkonen, J. Peränen, and L. Kääriäinen, “ATPase and GTPase activities associated with Semliki Forest virus nonstructural protein nsP2,” Journal of Virology, vol. 68, no. 9, pp. 5804–5810, 1994.
- L. Vasiljeva, A. Merits, P. Auvinen, and L. Kääriäinen, “Identification of a novel function of the Alphavirus capping apparatus. RNA 5'-triphosphatase activity of Nsp2,” The Journal of Biological Chemistry, vol. 275, no. 23, pp. 17281–17287, 2000.
- Y. S. Hahn, E. G. Strauss, and J. Strauss, “Mapping of RNA- temperature-sensitive mutants of Sindbis virus: assignment of complementation groups A, B, and G to nonstructural proteins,” Journal of Virology, vol. 63, no. 7, pp. 3142–3150, 1989.
- H. Malet, E. A. Gould, S. Jamal et al., “The crystal structures of Chikungunya and Venezuelan equine encephalitis virus nsP3 macro domains define a conserved adenosine binding pocket,” Journal of Virology, vol. 83, no. 13, pp. 6534–6545, 2009.
- E. Park and D. E. Griffin, “The nsP3 macro domain is important for Sindbis virus replication in neurons and neurovirulence in mice,” Virology, vol. 388, no. 2, pp. 305–314, 2009.
- M. T. Tuittila and A. Hinkkanen, “Amino acid mutations in the replicase protein nsP3 of Semliki Forest virus cumulatively affect neurovirulence,” Journal of General Virology, vol. 84, no. 6, pp. 1525–1533, 2003.
- Y. S. Hahn, A. Grakoui, C. M. Rice, E. G. Strauss, and J. Strauss, “Mapping of RNA- temperature-sensitive mutants of Sindbis virus: complementation group F mutants have lesions in nsP4,” Journal of Virology, vol. 63, no. 3, pp. 1194–1202, 1989.
- Y. Shirako, E. G. Strauss, and J. Strauss, “Suppressor mutations that allow Sindbis virus RNA polymerase to function with nonaromatic amino acids at the N-terminus: evidence for interaction between nsP1 and nsP4 in minus-strand RNA synthesis,” Virology, vol. 276, no. 1, pp. 148–160, 2000.
- S. Tomar, R. W. Hardy, J. L. Smith, and R. J. Kuhn, “Catalytic core of alphavirus nonstructural protein nsP4 possesses terminal adenylyltransferase activity,” Journal of Virology, vol. 80, no. 20, pp. 9962–9969, 2006.
- K. E. Owen and R. J. Kuhn, “Identification of a region in the Sindbis virus nucleocapsid protein that is involved in specificity of RNA encapsidation,” Journal of Virology, vol. 70, no. 5, pp. 2757–2763, 1996.
- B. G. Weiss, H. Nitschko, I. R. Ghattas, S. Schlesinger, and R. N. Wright, “Evidence for specificity in the encapsidation of Sindbis virus RNAs,” Journal of Virology, vol. 63, no. 12, pp. 5310–5318, 1989.
- R. Perera, C. K. Navaratnarajah, and R. J. Kuhn, “A heterologous coiled coil can substitute for helix I of the Sindbis virus capsid protein,” Journal of Virology, vol. 77, no. 15, pp. 8345–8353, 2003.
- H. K. Choi, L. Tong, W. Minor, et al., “Structure of Sindbis virus core protein reveals a chymotrypsin-like serine proteinase and the organization of the virion,” Nature, vol. 353, no. 6348, pp. 37–43, 1991.
- H. Garoff, K. Simons, and O. Renkonen, “Isolation and characterization of the membrane proteins of Semliki Forest virus,” Virology, vol. 61, no. 2, pp. 493–504, 1974.
- B. Simizu, K. Yamamoto, K. Hashimoto, and T. Ogata, “Structural proteins of Chikungunya virus,” Journal of Virology, vol. 51, no. 1, pp. 254–258, 1984.
- H. Garoff, A. M. Frischauf, and K. Simons, “Nucleotide sequence of cDNA coding for Semliki Forest virus membrane glycoproteins,” Nature, vol. 288, no. 5788, pp. 236–241, 1980.
- N. Liu and D. T. Brown, “Transient translocation of the cytoplasmic (endo) domain of a type I membrane glycoprotein into cellular membranes,” Journal of Cell Biology, vol. 120, no. 4, pp. 877–883, 1993.
- T. Smith, R. H. Cheng, N. Olson, et al., “Putative receptor binding sites on alphaviruses as visualized by cryoelectron microscopy,” Proceedings of the National Academy of Sciences of the United States of America, vol. 92, no. 23, pp. 10648–10652, 1995.
- N. L. Davis, F. J. Fuller, and W. G. Dougherty, “A single nucleotide change in the E2 glycoprotein gene of Sindbis virus affects penetration rate in cell culture and virulence in neonatal mice,” Proceedings of the National Academy of Sciences of the United States of America, vol. 83, no. 18, pp. 6771–6775, 1986.
- P. C. Tucker, S. H. Lee, N. Bui, D. E. Griffin, and D. Martinie, “Amino acid changes in the sindbis virus E2 glycoprotein that increase neurovirulence improve entry into neuroblastoma cells,” Journal of Virology, vol. 71, no. 8, pp. 6106–6112, 1997.
- S. Ubol, P. C. Tucker, D. E. Griffin, and J. M. Hardwick, “Neurovirulent strains of Alphavirus induce apoptosis in bcl-2-expressing cells: role of a single amino acid change in the E2 glycoprotein,” Proceedings of the National Academy of Sciences of the United States of America, vol. 91, no. 11, pp. 5202–5206, 1994.
- U. Skoging-Nyberg and P. Liljeström, “A conserved leucine in the cytoplasmic domain of the Semliki Forest virus spike protein is important for budding,” Archives of Virology, vol. 145, no. 6, pp. 1225–1230, 2000.
- L. Ivanova and M. Schlesinger, “Site-directed mutations in the Sindbis virus E2 glycoprotein identify palmitoylation sites and affect virus budding,” Journal of Virology, vol. 67, no. 5, pp. 2546–2551, 1993.
- K. Gaedigk-Nitschko and M. J. Schlesinger, “The Sindbis virus 6K protein can be detected in virions and is acylated with fatty acids,” Virology, vol. 175, no. 1, pp. 274–281, 1990.
- L. Ivanova, L. D. Le, and M. J. Schlesinger, “Characterization of revertants of a Sindbis virus 6K gene mutant that affects proteolytic processing and virus assembly,” Virus Research, vol. 39, no. 2-3, pp. 165–179, 1995.
- S. Lusa, H. Garoff, and P. Liljeström, “Fate of the 6K membrane protein of Semliki Forest virus during virus assembly,” Virology, vol. 185, no. 2, pp. 843–846, 1991.
- M. A. Sanz and L. Carrasco, “Sindbis virus variant with a deletion in the 6K gene shows defects in glycoprotein processing and trafficking: lack of complementation by a wild-type 6K gene in trans,” Journal of Virology, vol. 75, no. 16, pp. 7778–7784, 2001.
- J. Melton, G. Ewart, R. C. Weir, E. K. Lee, P. G. Board, and P. W. Gage, “Alphavirus 6K proteins form ion channels,” The Journal of Biological Chemistry, vol. 277, no. 49, pp. 46923–46931, 2002.
- M. A. Sanz, V. Madan, L. Carrasco, and J. L. Nieva, “Interfacial domains in sindbis virus 6K protein: detection and functional characterization,” The Journal of Biological Chemistry, vol. 278, no. 3, pp. 2051–2057, 2003.
- M. A. Sanz, L. G. Perez, and L. Carrasco, “Semliki forest virus 6K protein modifies membrane permeability after inducible expression in Escherichia coli cells,” The Journal of Biological Chemistry, vol. 269, no. 16, pp. 12106–12110, 1994.
- W. M. Boggs, C. S. Hahn, E. G. Strauss, J. Strauss, and D. E. Griffin, “Low pH-dependent Sindbis virus-induced fusion of BHK cells: differences between strains correlate with amino acid changes in the E1 glycoprotein,” Virology, vol. 169, no. 2, pp. 485–488, 1989.
- A. Omar and H. Koblet, “Semliki Forest virus particles containing only the E1 envelope glycoprotein are infectious and can induce cell-cell fusion,” Virology, vol. 166, no. 1, pp. 17–23, 1988.
- N. T. Glanville, M. Ranki, and J. Morser, “Initiation of translocation directed by 42S and 26S RNAs from Semliki Forest virus in vitro,” Proceedings of the National Academy of Sciences of the United States of America, vol. 73, no. 9, pp. 3059–3063, 1976.
- R. J. de Groot, W. R. Hardy, Y. Shrako, and J. Strauss, “Cleavage-site preferences of Sindbis virus polyproteins contains the non-structural proteinase. Evidence for temporal regulation of polyprotein processing in vivo,” The EMBO Journal, vol. 9, no. 8, pp. 2631–2638, 1990.
- K. Takkinen, J. Peränen, and L. Kääriäinen, “Proteolytic processing of Semliki Forest virus-specific non-structural polyprotein,” Journal of General Virology, vol. 72, no. 7, pp. 1627–1633, 1991.
- J. A. Lemm, T. Rümenapf, E. G. Strauss, C. M. Rice, and J. Strauss, “Polypeptide requirements for assembly of functional Sindbis virus replication complexes: a model for the temporal regulation of minus- and plus-strand RNA synthesis,” The EMBO Journal, vol. 13, no. 12, pp. 2925–2934, 1994.
- Y. Shirako and J. Strauss, “Regulation of Sindbis virus RNA replication: uncleaved P123 and nsP4 function in minus-strand RNA synthesis, whereas cleaved products from P123 are required for efficient plus-strand RNA synthesis,” Journal of Virology, vol. 68, no. 3, pp. 1874–1885, 1994.
- I. Dé, S. G. Sawicki, and D. L. Sawicki, “Sindbis virus RNA-negative mutants that fail to convert from minus-strand to plus-strand synthesis: role of the nsP2 protein,” Journal of Virology, vol. 70, no. 5, pp. 2706–2719, 1996.
- S. A. Froshauer, J. Kartenbeck, and A. Helenius, “Alphavirus RNA replicase is located on the cytoplasmic surface of endosomes and lysosomes,” Journal of Cell Biology, vol. 107, no. 6, pp. 2075–2086, 1988.
- P. Kujala, A. Ikäheimonen, N. Ehsani, H. Vihinen, L. Kääriäinen, and P. Auvinen, “Biogenesis of the Semliki Forest virus RNA replication complex,” Journal of Virology, vol. 75, no. 8, pp. 3873–3884, 2001.
- A. H. Salonen, L. Vasiljeva, A. Merits, L. Kääriäinen, J. Magden, and E. Jokitalo, “Properly folded nonstructural polyprotein directs the semliki forest virus replication complex to the endosomal compartment,” Journal of Virology, vol. 77, no. 3, pp. 1691–1702, 2003.
- M. W. LaStarza, J. A. Lemm, and C. M. Rice, “Genetic analysis of the nsP3 region of Sindbis virus: evidence for roles in minus-strand and subgenomic RNA synthesis,” Journal of Virology, vol. 68, no. 9, pp. 5781–5791, 1994.
- G. Li and C. M. Rice, “The signal for translational readthrough of a UGA codon in sindbis virus RNA involves a single cytidine residue immediately downstream of the termination codon,” Journal of Virology, vol. 67, no. 8, pp. 5062–5067, 1993.
- R. J. de Groot, T. Rümenapf, R. J. Kuhn, E. G. Strauss, and J. Strauss, “Sindbis virus RNA polymerase is degraded by the N-end rule pathway,” Proceedings of the National Academy of Sciences of the United States of America, vol. 88, no. 20, pp. 8967–8971, 1991.
- Y. Shirako and J. Strauss, “Requirement for an aromatic amino acid or histidine at the N terminus of sindbis virus RNA polymerase,” Journal of Virology, vol. 72, no. 3, pp. 2310–2315, 1998.
- G. Aliperti and M. Schlesinger, “Evidence for an autoprotease activity of Sindbis virus capsid protein,” Virology, vol. 90, no. 2, pp. 366–369, 1978.
- H. Garoff, K. Simons, and B. Dobberstein, “Assembly of the semliki forest virus membrane glycoproteins in the membrane of the endoplasmic reticulum in vitro,” Journal of Molecular Biology, vol. 124, no. 5, pp. 587–600, 1978.
- M. Lobigs, Z. Hongxing, and H. Garoff, “Function of Semliki Forest virus E3 peptide in virus assembly: replacement of E3 with an artificial signal peptide abolishes spike heterodimerization and surface expression of E1,” Journal of Virology, vol. 64, no. 9, pp. 4346–4355, 1990.
- W. A. Duffus, P. Levy-Mintz, M. R. Klimjack, and M. Kielian, “Mutations in the putative fusion peptide of Semliki Forest virus affect spike protein oligomerization and virus assembly,” Journal of Virology, vol. 69, no. 4, pp. 2471–2479, 1995.
- H. H. Andersson, B. U. Barth, M. Ekström, and H. Garoff, “Oligomerization-dependent folding of the membrane fusion protein of semliki forest virus,” Journal of Virology, vol. 71, no. 12, pp. 9654–9663, 1997.
- B. U. Barth, J. M. Wahlberg, and H. Garoff, “The oligomerization reaction of the Semliki forest virus membrane protein subunits,” Journal of Cell Biology, vol. 128, no. 3, pp. 283–291, 1995.
- I. de Curtis and K. Simons, “Dissection of Semliki Forest virus glycoprotein delivery from the trans-Golgi network to the cell surface in permeabilized BHK cells,” Proceedings of the National Academy of Sciences of the United States of America, vol. 85, no. 21, pp. 8052–8056, 1988.
- J. Green, G. Griffiths, and D. Louvard, “Passage of viral membrane proteins through the Golgi complex,” Journal of Molecular Biology, vol. 152, no. 4, pp. 663–698, 1981.
- M. Sariola, J. Saraste, and E. Kuismanen, “Communication of post-Golgi elements with early endocytic pathway: regulation of endoproteolytic cleavage of Semliki Forest virus p62 precursor,” Journal of Cell Science, vol. 108, part 6, pp. 2465–2475, 1995.
- A. Ziemiecki, H. Garoff, and K. Simons, “Formation of the Semliki Forest virus membrane glycoprotein complexes in the infected cell,” Journal of General Virology, vol. 50, no. 1, pp. 111–123, 1980.
- M. Lobigs and H. Garoff, “Fusion function of the Semliki Forest virus spike is activated by proteolytic cleavage of the envelope glycoprotein precursor p62,” Journal of Virology, vol. 64, no. 3, pp. 1233–1240, 1990.
- K. Metsikkö and H. Garoff, “Oligomers of the cytoplasmic domain of the p62/E2 membrane protein of Semliki Forest virus bind to the nucleocapsid in vitro,” Journal of Virology, vol. 64, no. 10, pp. 4678–4683, 1990.
- D. Vaux, A. Helenius, and I. Mellman, “Spike-nucleocapsid interaction in Semliki Forest virus reconstructed using network antibodies,” Nature, vol. 336, no. 6194, pp. 36–42, 1988.
- S. Mayor and R. E. Pagano, “Pathways of clathrin-independent endocytosis,” Nature Reviews Molecular Cell Biology, vol. 8, no. 8, pp. 603–612, 2007.
- A. L. Smith and G. H. Tignor, “Host cell receptors for two strains of Sindbis virus,” Archives of Virology, vol. 66, no. 1, pp. 11–26, 1980.
- K. S. Wang, A. L. Schmaljohn, R. J. Kuhn, and J. Strauss, “Antiidiotypic antibodies as probes for the Sindbis virus receptor,” Virology, vol. 181, no. 2, pp. 694–702, 1991.
- K. S. Wang, R. J. Kuhn, E. G. Strauss, J. Strauss, and S. Ou, “High-affinity laminin receptor is a receptor for Sindbis virus in mammalian cells,” Journal of Virology, vol. 66, no. 8, pp. 4992–5001, 1992.
- W. B. Klimstra, E. M. Nangle, M. S. Smith, A. D. Yurochko, and K. D. Ryman, “DC-SIGN and L-SIGN can act as attachment receptors for alphaviruses and distinguish between mosquito cell- and mammalian cell-derived viruses,” Journal of Virology, vol. 77, no. 22, pp. 12022–12032, 2003.
- K. Bernard, W. B. Klimstra, and R. E. Johnston, “Mutations in the E2 glycoprotein of Venezuelan equine encephalitis virus confer heparan sulfate interaction, low morbidity, and rapid clearance from blood of mice,” Virology, vol. 276, no. 1, pp. 93–103, 2000.
- M. Heil, A. Albee, J. Strauss, and R. J. Kuhn, “An amino acid substitution in the coding region of the E2 glycoprotein adapts ross river virus to utilize heparan sulfate as an attachment moiety,” Journal of Virology, vol. 75, no. 14, pp. 6303–6309, 2001.
- W. B. Klimstra, K. D. Ryman, and R. E. Johnston, “Adaptation of Sindbis virus to BHK cells selects for use of heparan sulfate as an attachment receptor,” Journal of Virology, vol. 72, no. 9, pp. 7357–7366, 1998.
- L. DeTulleo and T. Kirchhausen, “The clathrin endocytic pathway in viral infection,” The EMBO Journal, vol. 17, no. 16, pp. 4585–4593, 1998.
- S. Doxsey, F. M. Brodsky, G. Blank, and A. Helenius, “Inhibition of endocytosis by anti-clathrin antibodies,” Cell, vol. 50, no. 3, pp. 453–463, 1987.
- A. Helenius, J. Kartenbeck, K. Simons, and E. Fries, “On the entry of Semliki Forest virus into BHK-21 cells,” Journal of Cell Biology, vol. 84, no. 2, pp. 404–420, 1980.
- A. M. van der Bliek, T. Redelmeier, H. Damke, S. L. Schmid, E. M. Meyerowitz, and E. J. Tisdale, “Mutations in human dynamin block an intermediate stage in coated vesicle formation,” Journal of Cell Biology, vol. 122, no. 3, pp. 553–563, 1993.
- T. Baba, H. Damke, J. E. Hinshaw, D. Warnock, K. Ikeda, and S. L. Schmid, “Role of dynamin in clathrin-coated vesicle formation,” Cold Spring Harbor Symposia on Quantitative Biology, vol. 60, pp. 235–242, 1995.
- H. Damke, T. Baba, D. Warnock, and S. L. Schmid, “Induction of mutant dynamin specifically blocks endocytic coated vesicle formation,” Journal of Cell Biology, vol. 127, no. 4, pp. 915–934, 1994.
- M. Sourisseau, C. Schilte, N. Casartelli, et al., “Characterization of reemerging chikungunya virus,” PLoS Pathogens, vol. 3, no. 6, article e89, 2007.
- A. A. Kolokoltsov, E. H. Fleming, and R. A. Davey, “Venezuelan equine encephalitis virus entry mechanism requires late endosome formation and resists cell membrane cholesterol depletion,” Virology, vol. 347, no. 2, pp. 333–342, 2006.
- C. Bucci, R. G. Parton, I. H. Mather et al., “The small GTPase rab5 functions as a regulatory factor in the early endocytic pathway,” Cell, vol. 70, no. 5, pp. 715–728, 1992.
- C. Bucci, P. D. Thomsen, P. Nicoziani, J. McCarthy, and B. Van Deurs, “Rab7: a key to lysosome biogenesis,” Molecular Biology of the Cell, vol. 11, no. 2, pp. 467–480, 2000.
- T. M. Colpitts, A. Moore, A. A. Kolokoltsov, and R. A. Davey, “Venezuelan equine encephalitis virus infection of mosquito cells requires acidification as well as mosquito homologs of the endocytic proteins Rab5 and Rab7,” Virology, vol. 369, no. 1, pp. 78–91, 2007.
- S. Cassell, J. Edwards, and D. T. Brown, “Effects of lysosomotropic weak bases on infection of BHK-21 cells by Sindbis virus,” Journal of Virology, vol. 52, no. 3, pp. 857–864, 1984.
- K. Coombs, E. Mann, J. Edwards, and D. T. Brown, “Effects of chloroquine and cytochalasin B on the infection of cells by Sindbis virus and vesicular stomatitis virus,” Journal of Virology, vol. 37, no. 3, pp. 1060–1065, 1981.
- R. Hernandez, T. Luo, and D. T. Brown, “Exposure to low pH is not required for penetration of mosquito cells by sindbis virus,” Journal of Virology, vol. 75, no. 4, pp. 2010–2013, 2001.
- A. Paredes, D. F. Ferreira, M. Horton, et al., “Conformational changes in Sindbis virions resulting from exposure to low pH and interactions with cells suggest that cell penetration may occur at the cell surface in the absence of membrane fusion,” Virology, vol. 324, no. 2, pp. 373–386, 2004.
- G. Wang, R. Hernandez, K. R. Weninger, and D. T. Brown, “Infection of cells by Sindbis virus at low temperature,” Virology, vol. 362, no. 2, pp. 461–467, 2007.
- M. Marsh and R. Bron, “SFV infection in CHO cells: cell-type specific restrictions to productive virus entry at the cell surface,” Journal of Cell Science, vol. 110, part 1, pp. 95–103, 1997.
- T. Hase, P. L. Summers, and W. H. Cohen, “A comparative study of entry modes into C 6/36 cells by Semliki Forest Japanese encephalitis viruses,” Archives of Virology, vol. 108, no. 1-2, pp. 101–114, 1989.
- E. A. Bernard, M. Solignat, B. Gay et al., “Endocytosis of chikungunya virus into mammalian cells: role of clathrin and early endosomal compartments,” PLoS One, vol. 5, no. 7, Article ID e11479, 2010.
- M. Kielian and A. Helenius, “pH-Iinduced alterations in the fusogenic spike protein of Semliki Forest virus,” Journal of Cell Biology, vol. 101, no. 6, pp. 2284–2291, 1985.
- J. Justman, M. R. Klimjack, and M. Kielian, “Role of spike protein conformational changes in fusion of Semliki Forest virus,” Journal of Virology, vol. 67, no. 12, pp. 7597–7607, 1993.
- M. A. Sanz, M. T. Rejas, and L. Carrasco, “Individual expression of Sindbis virus glycoproteins. E1 alone promotes cell fusion,” Virology, vol. 305, no. 2, pp. 463–472, 2003.
- C. Spyr, F. Käsermann, and C. Kempf, “Identification of the pore forming element of Semliki Forest virus spikes,” The FEBS Letters, vol. 375, no. 1-2, pp. 134–136, 1995.
- R. Bron, J. Wahlberg, H. Garoff, and J. Wilschut, “Membrane fusion of Semliki Forest virus in a model system: correlation between fusion kinetics and structural changes in the envelope glycoprotein,” The EMBO Journal, vol. 12, no. 2, pp. 693–701, 1993.
- M. R. Klimjack, S. Jeffrey, and M. Kielian, “Membrane and protein interactions of a soluble form of the Semliki Forest virus fusion protein,” Journal of Virology, vol. 68, no. 11, pp. 6940–6946, 1994.
- J. M. Smit, R. Bittman, and J. Wilschut, “Low-pH-dependent fusion of Sindbis virus with receptor-free cholesterol- and sphingolipid-containing liposomes,” Journal of Virology, vol. 73, no. 10, pp. 8476–8484, 1999.
- J. White and A. Helenius, “pH-Dependent fusion between the Semliki Forest virus membrane and liposomes,” Proceedings of the National Academy of Sciences of the United States of America, vol. 77, no. 6, pp. 3273–3277, 1980.
- J. Wahlberg, R. Bron, J. Wilschut, and H. Garoff, “Membrane fusion of Semliki Forest virus involves homotrimers of the fusion protein,” Journal of Virology, vol. 66, no. 12, pp. 7309–7318, 1992.
- J. Wahlberg and H. Garoff, “Membrane fusion process of Semliki Forest virus I: low pH-induced rearrangement in spike protein quaternary structure precedes virus penetration into cells,” Journal of Cell Biology, vol. 116, no. 2, pp. 339–348, 1992.
- M. Lanzrein, R. Weingart, and C. Kempf, “PH-dependent pore formation in Semliki forest virus-infected Aedes albopictus cells,” Virology, vol. 193, no. 1, pp. 296–302, 1993.
- A. Schlegel, A. Omar, P. Jentsch, C. Kempf, and A. Morell, “Semliki Forest virus envelope proteins function as proton channels,” Bioscience Reports, vol. 11, no. 5, pp. 243–255, 1991.
- A. Helenius, M. Marsh, and J. M. White, “Inhibition of Semliki Forest virus penetration by lysosomotropic weak bases,” Journal of General Virology, vol. 58, no. 1, pp. 47–61, 1982.
- M. Marsh, J. Wellsteed, and H. Kern, “Monensin inhibits Semliki Forest virus penetration into culture cells,” Proceedings of the National Academy of Sciences of the United States of America, vol. 79, no. 17, pp. 5297–5301, 1982.
- T. Phalen and M. Kielian, “Cholesterol is required for infection by Semliki Forest virus,” Journal of Cell Biology, vol. 112, no. 4, pp. 615–623, 1991.
- J. Wilschut, J. Corver, J. L. Nieva, et al., “Fusion of Semliki Forest virus with cholesterol-containing liposomes at low pH: a specific requirement for sphingolipids,” Molecular Membrane Biology, vol. 12, no. 1, pp. 143–149, 1995.
- A. Ahn, D. L. Gibbons, and M. Kielian, “The fusion peptide of Semliki Forest virus associates with sterol-rich membrane domains,” Journal of Virology, vol. 76, no. 7, pp. 3267–3275, 2002.
- Y. E. Lu, T. Cassese, and M. Kielian, “The cholesterol requirement for sindbis virus entry and exit and characterization of a spike protein region involved in cholesterol dependence,” Journal of Virology, vol. 73, no. 5, pp. 4272–4278, 1999.
- J. L. Nieva, R. Bron, J. Corver, and J. Wilschut, “Membrane fusion of Semliki Forest virus requires sphingolipids in the target membrane,” The EMBO Journal, vol. 13, no. 12, pp. 2797–2804, 1994.
- M. Kielian and A. Helenius, “Role of cholesterol in fusion of Semliki Forest virus with membranes,” Journal of Virology, vol. 52, no. 1, pp. 281–283, 1984.
- P. K. Chatterjee, M. Vashishtha, and M. Kielian, “Biochemical consequences of a mutation that controls the cholesterol dependence of Semliki Forest virus fusion,” Journal of Virology, vol. 74, no. 4, pp. 1623–1631, 2000.
- M. Vashishtha, T. Phalen, M. T. Marquardt, J. S. Ryu, M. Kielian, and A. C. Ng, “A single point mutation controls the cholesterol dependence of Semliki Forest virus entry and exit,” Journal of Cell Biology, vol. 140, no. 1, pp. 91–99, 1998.
- W. H. Evans and W. G. Hardison, “Phospholipid, cholesterol, polypeptide and glycoprotein composition of hepatic endosome subfractions,” Biochemical Journal, vol. 232, no. 1, pp. 33–36, 1985.
- T. Kobayashi, M. H. Beuchat, J. Chevallier, et al., “Separation and characterization of late endosomal membrane domains,” The Journal of Biological Chemistry, vol. 277, no. 35, pp. 32157–32164, 2002.
- G. Wengler, A. Koschinski, G. Wengler, and H. Repp, “During entry of alphaviruses, the E1 glycoprotein molecules probably form two separate populations that generate either a fusion pore or ion-permeable pores,” Journal of General Virology, vol. 85, no. 6, pp. 1695–1701, 2004.
- K. C. Duff and R. H. Ashley, “The transmembrane domain of influenza A M2 protein forms amantadine- sensitive proton channels in planar lipid bilayers,” Virology, vol. 190, no. 1, pp. 485–489, 1992.
- M. Dick, B. U. Barth, and C. Kempf, “The E1 protein is mandatory for pore formation by Semliki Forest virus spikes,” Virology, vol. 220, no. 1, pp. 204–207, 1996.
- G. Wengler, A. Koschinski, G. Wengler, and F. Dreyer, “Entry of alphaviruses at the plasma membrane converts the viral surface proteins into an ionpermeable pore that can be detected by electrophysiological analyses of whole-cell membrane currents,” Journal of General Virology, vol. 84, no. 1, pp. 173–181, 2003.
- G. Wengler and G. Wengler, “In vitro analysis of factors involved in the disassembly of Sindbis virus cores by 60S ribosomal subunits identifies a possible role of low pH,” Journal of General Virology, vol. 83, part 10, pp. 2417–2426, 2002.
- I. R. Singh and A. Helenius, “Role of ribosomes in Semliki Forest virus nucleocapsid uncoating,” Journal of Virology, vol. 66, no. 12, pp. 7049–7058, 1992.
- I. Ulmanen, H. Söderlund, and L. Kääriäinen, “Semliki forest virus capsid protein associates with the 60S ribosomal subunit in infected cells,” Journal of Virology, vol. 20, no. 1, pp. 203–210, 1976.
- G. Wengler, D. Wurkner, and G. Wengler, “Identification of a sequence element in the alphavirus core protein which mediates interaction of cores with ribosomes and the disassembly of cores,” Virology, vol. 191, no. 2, pp. 880–888, 1992.
- M. Solignat, B. Gay, S. Higgs, et al., “Replication cycle of chikungunya: a re-emerging arbovirus,” Virology, vol. 393, no. 2, pp. 183–197, 2009.
- C. Lamaze, A. Dujeancourt, T. Baba, et al., “Interleukin 2 receptors and detergent-resistant membrane domains define a clathrin-independent endocytic pathway,” Molecular Cell, vol. 7, no. 3, pp. 661–671, 2001.